Dataset Information


Concentration dependent gene expression changes in Daphnia magna exposed to metals

ABSTRACT: To determine dose-dependent effects of metal exposure we performed microarray hybridizations with RNA isolated from Daphnia magna following Cu, Cd, and Zn exposures over a range of concentrations that included a tolerated concentration, a sublethal concentration, and a highly toxic concentration. The gene expression profiles revealed effects to digestion related genes, immune related genes, metallothioneins, and oxidative stress genes at the higher concentrations. We also observed that the highest concentrations produced less specific gene expression profiles than the lower concentrations suggesting a more general stress response at the higher concentrations. The lowest concentration tested, representing tolerated concentrations of the metals, caused differential expression of only a few genes and were distinct from the expression profiles of the higher concentrations. This result provides support for the presence of a No Observed Transcriptional Effect Level (NOTEL) for metal exposure in D. magna and suggests that gene expression analysis may offer a strategy for distinguishing between toxic and nontoxic concentrations of metals in the environment. Keywords: ecotoxicogenomic exposure study Overall design: We exposed Daphnia magna to 2 different concentrations of Cd and Zn and 3 different concentrations of Cu for 24 hours. For each exposure condition, we performed 3 exposures and 2 technical replicates (as dye swap) for each exposure (6 microarrays total). All exposures were compared to a unexposed laboratory control (COMBO media).

INSTRUMENT(S): Daphnia magna DGC cDNA array ver.1

SUBMITTER: Helen C Poynton  

PROVIDER: GSE7668 | GEO | 2008-06-09



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Gene expression profiling in Daphnia magna part I: concentration-dependent profiles provide support for the No Observed Transcriptional Effect Level.

Poynton Helen C HC   Loguinov Alexandre V AV   Varshavsky Julia R JR   Chan Sarah S   Perkins Edward J EJ   Vulpe Chris D CD  

Environmental science & technology 20080801 16

Ecotoxicogenomic approaches to environmental monitoring provide holistic information, offer insight into modes of action, and help to assess the causal agents and potential toxicity of effluents beyond the traditional end points of death and reproduction. Recent investigations of toxicant exposure indicate dose-dependent changes are a key issue in interpreting genomic studies. Additionally, there is interest in developing methods to integrate gene expression studies in environmental monitoring a  ...[more]

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