Genomics

Dataset Information

162

Genome-wide distribution of proteins Pita, ZIPIC, Zw5 in Drosophila embryos


ABSTRACT: According to recent models, as yet poorly studied architectural proteins appear to be required for local regulation of enhancer–promoter interactions, as well as for global chromosome organization. Transcription factors ZIPIC, Pita, and Zw5 belong to the class of chromatin insulator proteins and preferentially bind to promoters near the TSS. They are structurally similar in containing the N-terminal zinc finger-associated domain (ZAD) and different numbers of C2H2-type zinc fingers at the C-terminus. We have shown that the ZAD domains of ZIPIC, Pita, and Zw5 usually form homodimers. In Drosophila transgenic lines, these proteins can support long-distance interaction between GAL4 activator and the reporter gene promoter. However, no functional interaction between binding sites for different proteins has been revealed, suggesting that such interactions are highly specific. ZIPIC facilitates long-distance stimulation of reporter gene by GAL4 activator in yeast. Thus, ZAD-containing zinc-finger proteins probably belong to the class of architectural proteins. Overall design: ChIP-Seq for Pita, ZIPIC, Zw5 proteins from Drosophila embryos

INSTRUMENT(S): Illumina HiSeq 2000 (Drosophila melanogaster)

SUBMITTER: Pavel Georgiev 

PROVIDER: GSE76997 | GEO | 2016-05-06

SECONDARY ACCESSION(S): PRJNA309199

REPOSITORIES: GEO

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Publications

Architectural proteins Pita, Zw5,and ZIPIC contain homodimerization domain and support specific long-range interactions in Drosophila.

Zolotarev Nikolay N   Fedotova Anna A   Kyrchanova Olga O   Bonchuk Artem A   Penin Aleksey A AA   Lando Andrey S AS   Eliseeva Irina A IA   Kulakovskiy Ivan V IV   Maksimenko Oksana O   Georgiev Pavel P  

Nucleic acids research 20160502 15


According to recent models, as yet poorly studied architectural proteins appear to be required for local regulation of enhancer-promoter interactions, as well as for global chromosome organization. Transcription factors ZIPIC, Pita and Zw5 belong to the class of chromatin insulator proteins and preferentially bind to promoters near the TSS and extensively colocalize with cohesin and condensin complexes. ZIPIC, Pita and Zw5 are structurally similar in containing the N-terminal zinc finger-associa  ...[more]

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