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Microarray analysis of CD4+T cells in murine arthritis

ABSTRACT: Although activated CD4+ T cell-driven overproduction of cytokines, especially TNF and IL1, is generally regarded as the major factor in the development of rheumatoid arthritis (RA), little is known about the precise role of CD4+ T cells in the initiation and progression of this disease. In this study, this issue was addressed using a time-course microarray analysis in a mouse model of RA, Il1rn deficient mice.No obvious cytokine gene expression changes reflecting T cell activation was observed in CD4+ T cells in the Il1rn deficient mice during the course of spontaneous arthritis. On the contrast, majority of dysregulated genes were those predominantly expressed in myeloid lineage cells, suggesting T cell reprogramming involvement in arthritis development. Distinct gene expression patterns were identified for different stages of disease, including downregulated expression of immunoglobulin heavy chain constant region genes and increased expression of inflammatory genes in the early phase, and downregulation of MHC class II genes in the late phase. The common changes occurred in both early and late phases included upregulation of Arl2bp and Mfap1, which are involved in the regulation of cytoskeletal dynamics. Keywords: Time course

ORGANISM(S): Mus musculus

PROVIDER: GSE7705 | GEO | 2008/05/01

SECONDARY ACCESSION(S): PRJNA99423

REPOSITORIES: GEO

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Transcription profiling of mouse model of rheumatoid arthritis Il1rn deficient animal CD4+ T cells

Project description:Although activated CD4+ T cell-driven overproduction of cytokines, especially TNF and IL1, is generally regarded as the major factor in the development of rheumatoid arthritis (RA), little is known about the precise role of CD4+ T cells in the initiation and progression of this disease. In this study, this issue was addressed using a time-course microarray analysis in a mouse model of RA, Il1rn deficient mice.No obvious cytokine gene expression changes reflecting T cell activation was observed in CD4+ T cells in the Il1rn deficient mice during the course of spontaneous arthritis. On the contrast, majority of dysregulated genes were those predominantly expressed in myeloid lineage cells, suggesting T cell reprogramming involvement in arthritis development. Distinct gene expression patterns were identified for different stages of disease, including downregulated expression of immunoglobulin heavy chain constant region genes and increased expression of inflammatory genes in the early phase, and downregulation of MHC class II genes in the late phase. The common changes occurred in both early and late phases included upregulation of Arl2bp and Mfap1, which are involved in the regulation of cytoskeletal dynamics. Experiment Overall Design: Two KO mice and three WT mice for each time point (1 month and 4 month) were used for this study.

2008-06-15 | E-GEOD-7705 | biostudies-arrayexpress

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2008-06-15 | E-GEOD-7657 | biostudies-arrayexpress

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Project description:Rheumatoid arthritis (RA) is a heterogeneous disease with clinical and biological polymorphisms. However, little is known about baseline molecular variations among individual RA patients. The purpose of this study was to address this issue using F2 intercross mice generated from arthritis-prone BALB/c and arthritis-resistant DBA/1 mice deficient for interleukin 1 receptor antagonist (Il1rn). Two distinct subpopulations of arthritic mice were identified in the 38 mice studied. One subgroup of diseased mice was characterized by myeloid cell dominant inflammation, whereas the other was mainly associated with increased anti-apoptotic activities of inflammatory cells. Keywords: disease state analysis

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Transcription profiling of mouse F2 intercross mice generated from arthritis-prone BALB/c and arthritis-resistant DBA/1 mice deficient for interleukin 1 receptor antagonist (Il1rn)

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2015-12-31 | E-GEOD-64612 | biostudies-arrayexpress

Gene expression of human peripheral blood cells of patients with rheumatoid arthritis [dataset 2]

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