Transcriptomics,Genomics

Dataset Information

158

Genome-wide analysis of developmentally regulated microRNAs in Hami melon fruit


ABSTRACT: Background: MicroRNAs (miRNAs) represent a family of small endogenous, non-coding RNAs that play critical regulatory roles in plant growth, development, and environmental stress responses. Although Hami melon is an attractive model for valuable biological traits analysis, the role of miRNA action in the fruit development and ripening remains largely unknown. Here, we performed small RNA sequencing to investigate the Hami melon miRNA profiles at four fruit developmental stages Results: Small RNA sequencing yielded raw reads in eight libraries. miRNAs expression profiles were variable at different fruit developmental stages. The expression levels of five known miRNAs were validated by quantitative real-time PCR. Among the identified miRNAs, several miRNAs showed developmentally regulated and differentially expressed pattern during fruit development. Conclusions: Our results present a first comprehensive set of identification and characterization of Hami melon fruit miRNAs and their potential targets, which provide valuable basis for further research on the critical role of miRNAs in melon fruit development. Overall design: Eight samples, including eight Hami melon flesh mesocarp samples for two separate small RNA sequencing to obtain biological replicates.

INSTRUMENT(S): Illumina Genome Analyzer IIx (Cucumis melo)

SUBMITTER: Dong Chen 

PROVIDER: GSE77127 | GEO | 2017-09-06

SECONDARY ACCESSION(S): PRJNA309519

REPOSITORIES: GEO

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Publications

Genome-wide identification of Hami melon miRNAs with putative roles during fruit development.

Zhang Hong H   Yin Lan L   Wang Huaisong H   Wang Guangzhi G   Ma Xinli X   Li Meihua M   Wu Haibo H   Fu Qiushi Q   Zhang Yi Y   Yi Hongping H  

PloS one 20170724 7


MicroRNAs represent a family of small endogenous, non-coding RNAs that play critical regulatory roles in plant growth, development, and environmental stress responses. Hami melon is famous for its attractive flavor and excellent nutritional value, however, the mechanisms underlying the fruit development and ripening remains largely unknown. Here, we performed small RNA sequencing to investigate the roles of miRNAs during Hami melon fruit development. Two batches of flesh samples were collected a  ...[more]

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