Genomics

Dataset Information

0

The target spectrum of SdsR small RNA in Salmonella


ABSTRACT: Enteric model bacteria such as Escherichia coli and Salmonella enterica express hundreds of small non-coding RNAs (sRNAs), targets for most of which are yet unknown. Some of these sRNAs are remarkably well-conserved, indicating that that they serve cellular functions that go beyond the necessities of a single organism. One of these “core sRNA” of largely unknown functions is the abundant ~100-nucleotide SdsR sRNA which accumulates in stationary phase after transcription by the general stress σ-factor, σS. In Salmonella, SdsR was known to inhibit the synthesis of the species-specific porin, OmpD. However, the sdsR gene is present in almost all enterobacterial genomes, suggesting additional, more conserved targets of this sRNA must exist. Here, we have combined SdsR pulse-expression with whole genome transcriptomics to discover 18 previously unknown targets of SdsR. These new targets include mRNAs coding for physiologically important regulators such as the carbon regulator, Crp, the nucleoid-associated chaperone, StpA and the antibiotic resistance transporter, TolC. SdsR is processed by RNase E giving rise to two independent SdsR variants with distinct target spectra. While the overall physiological role of this orphan core sRNA remains to be fully understood, the here presented catalog of new SdsR targets contains valuable leads to understand sRNA functions in resting bacteria.

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 Salmonella enterica

PROVIDER: GSE77157 | GEO | 2016/01/25

SECONDARY ACCESSION(S): PRJNA309607

REPOSITORIES: GEO

Similar Datasets

2009-10-19 | GSE17771 | GEO
2014-05-05 | GSE46380 | GEO
| E-GEOD-16540 | biostudies-arrayexpress
| E-GEOD-67947 | biostudies-arrayexpress
| E-MTAB-329 | biostudies-arrayexpress
| E-GEOD-17771 | biostudies-arrayexpress
| E-GEOD-26573 | biostudies-arrayexpress
2024-02-08 | PXD046416 | Pride
2020-05-01 | GSE123049 | GEO
2020-05-01 | GSE123048 | GEO