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RecG Directs DNA Synthesis During Double-Strand Break Repair [ChIP-seq]

ABSTRACT: In the bacterium Escherichia coli, RecG directs DNA synthesis during the repair of DNA double-strand breaks by homologous recombination.

ORGANISM(S): Escherichia coli

PROVIDER: GSE77181 | GEO | 2016/05/19

SECONDARY ACCESSION(S): PRJNA309668

REPOSITORIES: GEO

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RecG Directs DNA Synthesis During Double-Strand Break Repair [ChIP-seq]

Project description:In the bacterium Escherichia coli, RecG directs DNA synthesis during the repair of DNA double-strand breaks by homologous recombination. Examination of RecA binding during double-strand break repair in Escherichia coli in the presence and absence of RecG protein

2016-05-19 | E-GEOD-77181 | biostudies-arrayexpress

RecG Directs DNA Synthesis During Double-Strand Break Repair [Chromosomal marker frequency analysis]

Project description:In the bacterium Escherichia coli, RecG directs DNA synthesis during the repair of DNA double-strand breaks by homologous recombination. Chromosomal marker frequency analysis (MFA) following induction of a DSB in the absence and presence of RecG

2016-05-19 | E-GEOD-77183 | biostudies-arrayexpress

RecG Directs DNA Synthesis During Double-Strand Break Repair [Chromosomal marker frequency analysis]

Project description:In the bacterium Escherichia coli, RecG directs DNA synthesis during the repair of DNA double-strand breaks by homologous recombination.

2016-05-19 | GSE77183 | GEO

RecG Directs DNA Synthesis During Double-Strand Break Repair

Project description:RecG Directs DNA Synthesis During Double-Strand Break Repair

| PRJNA309667 | ENA

RecG Directs DNA Synthesis During Double-Strand Break Repair

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-05-19 | E-GEOD-77184 | biostudies-arrayexpress

RecG Directs DNA Synthesis during Double-Strand Break Repair.

Project description: Not available

| S-EPMC4752480 | biostudies-literature

RecG Directs DNA Synthesis During Double-Strand Break Repair

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-05-19 | GSE77184 | GEO

CHD7- and HDAC1/2-dependent chromatin remodeling activities cooperate to promote DNA double-strand break repair

Project description:The DNA double-strand break (DSB) response is a multi-step process that requires chromatin reorganization at each stage. 53BP1 is a nodal point in this response as it directs chromatin-based DSB repair. However, how chromatin reorganization impacts DSB repair upstream of 53BP1 is not fully understood. Here we reveal that Chromodomain Helicase DNA Binding Protein 7 (CHD7) rapidly recruits to damaged chromatin in a poly(ADP-ribose) polymerase 1 (PARP1)-dependent manner. CHD7 facilitates chromatin relaxation and the loading of canonical non-homologous end-joining (cNHEJ) factors, while restraining 53BP1 accumulation at DSBs. Moreover, CHD7 physically associates with HDAC1/2, thereby recruiting this complex to DNA breaks independently of its chromatin remodeling activity. HDAC1/2 promote histone H4 de-acetylation and chromatin re-compaction to prevent supraphysiological retention of cNHEJ factors at DBSs. Thus, the cooperation between distinct yet coordinated chromatin remodeling activities driven by CHD7 and HDAC1/2 ensures proper assembly dynamics of the cNHEJ machinery and efficient DSB repair.

2020-08-28 | PXD014339 | Pride

FOXL2 directs DNA double-strand break repair pathways by differentially interacting with Ku.

Project description: Not available

| S-EPMC7181608 | biostudies-literature

Monitoring Homology Search during DNA Double-Strand Break Repair in vivo

Project description:Homologous recombination (HR) is crucial for genetic exchange, accurate repair of DNA double-strand breaks and pivotal for genome integrity. HR uses homologous sequences for repair, but how homology search, the exploration of the genome for homologous DNA sequences, is conducted in the nucleus remains poorly understood. Here, we use time-resolved chromatin immunoprecipitations of repair proteins to monitor homology search in vivo. We found that homology search proceeds by a probing mechanism, which commences around the break and samples preferentially on the broken chromosome. However, elements thought to instruct chromosome loops mediate homology search shortcuts, and centromeres, which cluster within the nucleus, may facilitate homology search on other chromosomes. Our study thus revealed crucial parameters for homology search in vivo and emphasizes the importance of linear distance, chromosome architecture and proximity for recombination efficiency. 2 new custom ChIP-chip platforms used; both Nimblegen; differ in oligo density: (platform 1: 2006-07-18_Scerevisiae_ChIP_Stefan Jentsch MPI Biochemistry S.cerevisiae 385K Tiling Array Version 1) ( platform 2: 100304_Scer2_MS_Chip_Stefan Jentsch MPI Biochemistry S.cerevisiae 135K Tiling Array Version 2) ChIP-chip profiling of DSB repair factors (Rad51, Rad52, RPA, gamma-H2A) upon single inducible DSBs in S.cerevisiae

2013-03-21 | E-GEOD-44844 | biostudies-arrayexpress

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