Genomics

Dataset Information

170

Chromosome Conformation of Human Fibroblasts Grown in 3-Dimensional Spheroids


ABSTRACT: In the study of interphase chromosome organization, genome-wide chromosome conformation capture (Hi-C) maps are often generated using 2-dimensional (2D) monolayer cultures. These 2D cells have morphological deviations from cells that exist in 3-dimensional (3D) tissues in vivo, and may not maintain the same chromosome conformation. We used Hi-C maps to test the extent of differences in chromosome conformation between human fibroblasts grown in 2D cultures and those grown in 3D spheroids. Significant differences in chromosome conformation were found between 2D cells and those grown in spheroids. Intra-chromosomal interactions were generally increased in spheroid cells, with a few exceptions, while inter-chromosomal interactions were generally decreased. Overall, chromosomes located closer to the nuclear periphery had increased intra-chromosomal contacts in spheroid cells, while those located more centrally had decreased interactions. This study highlights the necessity to conduct studies on the topography of the interphase nucleus under conditions that mimic an in vivo environment. Overall design: Genome wide chromosome conformation capture (Hi-C) data from primary human fibroblasts grown in 2D and 3D plates, 2 replicates

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)

SUBMITTER: Indika Rajapakse  

PROVIDER: GSE77237 | GEO | 2016-01-26

SECONDARY ACCESSION(S): PRJNA309829

REPOSITORIES: GEO

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Publications

Chromosome conformation of human fibroblasts grown in 3-dimensional spheroids.

Chen Haiming H   Comment Nicholas N   Chen Jie J   Ronquist Scott S   Hero Alfred A   Ried Thomas T   Rajapakse Indika I  

Nucleus (Austin, Tex.) 20150101 1


In the study of interphase chromosome organization, genome-wide chromosome conformation capture (Hi-C) maps are often generated using 2-dimensional (2D) monolayer cultures. These 2D cells have morphological deviations from cells that exist in 3-dimensional (3D) tissues in vivo, and may not maintain the same chromosome conformation. We used Hi-C maps to test the extent of differences in chromosome conformation between human fibroblasts grown in 2D cultures and those grown in 3D spheroids. Signifi  ...[more]

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