Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq transcriptomic profiling for self-renewing (SR) and senescent (SEN) human adult adipose derived mesenchymal stem cells (hADSCs)

ABSTRACT: Characterization of gene expression levels with RNA-seq was performed on self-renewing (SR) and senescent (SEN) human adult adipose derived mesenchymal stem cells (hADSCs) using the Roche 454 pyrosequencing platform.

ORGANISM(S): Homo sapiens

PROVIDER: GSE77284 | GEO | 2017/08/15

SECONDARY ACCESSION(S): PRJNA309967

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

High-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment

Project description:Contaminated aquifer (Dusseldorf-Flinger, Germany) templates extracted from 5 sediment depths ranging between 6.4 and 8.4 m below ground and over 3 years of sampling were amplified for amplicon pyrosequencing using the primers Ba27f (5’-aga gtt tga tcm tgg ctc ag-3’) and Ba519r (5’- tat tac cgc ggc kgc tg-3’), extended as amplicon fusion primers with respective primer A or B adapters, key sequence and multiplex identifiers (MID) as recommended by 454/Roche. Amplicons were purified and pooled as specified by the manufacturer. Emulsion PCR (emPCR), purification of DNA-enriched beads and sequencing run were performed following protocols and using a 2nd generation pyrosequencer (454 GS FLX Titanium, Roche) as recommended by the developer. Quality filtering of the pyrosequencing reads was performed using the automatic amplicon pipeline of the GS Run Processor (Roche), with a slight modification concerning the valley filter (vfScanAllFlows false instead of TiOnly) to extract the sequences. Demultiplexed raw reads were furhter trimmed for quality and lenght (>250 bp).

2012-11-11 | GSE25345 | GEO

Metformin improves cognition of aged mice by promoting cerebral angiogenesis and neurogenesis [hADSCs]

Project description:We implemented transcriptomic analyses of hADSCs treated with metformin 6 days

2020-09-23 | GSE140714 | GEO

High-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment

2012-11-11 | E-GEOD-25345 | biostudies-arrayexpress

Project description:Transcriptome sequencing of Three Begonia species, using Roche 454 Titanium pyrosequencing.

| PRJEB2868 | ENA

HEP14-activated PKC-ERK1/2 pathway boosts HEP14-empowered hADSCs for ovarian regeneration and functional restoration

Project description:Ovarian insufficiency (OI), including both premature ovarian insufficiency (POI) and age-related natural-aging ovarian insufficiency (ARNA-OI), significantly affects women’s health and remains persistent challenge in clinical treatment. In this study, bulk and scRNA-sequencing analysis indicated that HEP14 alters gene expression patterns in hADSCs and promotes the survival, antifibrotic, and proangiogenic capabilities, as well as the transdifferentiation potential of hADSCs. The key factors involved in these processes were upregulated by the HEP14-activated PKC-ERK1/2 signaling pathway. In both doxorubicin-induced POI and age-related natural-aged ARNA-OI mouse models, the combined administration of h-hADSCs and HEP14 encapsulated in PLGA microspheres (HEP14/PLGA) substantially enhanced ovarian regeneration and restoration of gonadal hormone levels through autocrine, paracrine, or endocrine mechanisms. Importantly, this combined therapy markedly improved the fertility of POI mice. These results demonstrate that the therapeutic potential of this combined therapy for POI and ARNA-OI, opening new avenues for clinical research and treatment strategies.

2025-07-17 | GSE273950 | GEO

Identifying differential gene expression in TBT-treated dog whelk, Nucella lapillus

Project description:Imposex, the superimposition of male sexual characteristics in females, is caused by tributyltin (TBT) and provides one of the best ecological examples of anthropogenically-induced endocrine disruption in aquatic ecosystems. We used combinations of 454 Roche pyrosequencing and microarray technologies to understand the functional genomic basis of imposex in the prosobranch gastropod, Nucella lapillus, a recognised sentinel for TBT-induced imposex.

2012-06-01 | E-MTAB-709 | biostudies-arrayexpress

HEP14-activated PKC-ERK1/2 pathway boosts empowered-hADSCs for ovarian regeneration and functional restoration [RNA-seq]

2025-07-17 | GSE273949 | GEO

Licochalcone A activate glycolysis by via PI3K / AKT pathway to play an anti-aging effect on adipose stem cells and aging mice [hADSCs]

Project description:We implemented transcriptomic analyses of hADSCs treated with Licochalcone A 6 days

2022-01-21 | GSE144122 | GEO

Homo sapiens

Project description:RNA-seq transcriptomic profiling for self-renewing (SR) and senescent (SEN) human adult adipose derived mesenchymal stem cells (hADSCs)

| PRJNA309967 | ENA

Expression data from RH30 IκBα SR cells and RH30 IkBa SR MyoD Crispr cells

Project description:Classical NF-κB activity can be inhibited by overexpression of the IκBα super repressor (SR). To determine the role of MyoD in rhabdomyosarcoma cells with NF-kB inhibited, ablated MyoD expression with CRISPR/cas9 editing and clonal selection of RH30-SR cell lines to compare differences in expression following deletion

2025-07-28 | GSE224171 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data