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Pho and Ph, H3K27me3 and Engrailed ChIP-seq data in PRE-deleted third instar Drosophila larval brains and disks and 4C-seq data in the third instar larval brains and disks

ABSTRACT: Genome-wide binding profile of PcG proteins- Pho and Ph, H3K27me3 and Engrailed in Drosophila third instar larval brains and disks, in duplicate, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE77341 | GEO | 2016/09/24

SECONDARY ACCESSION(S): PRJNA310099

REPOSITORIES: GEO

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Pho and Ph, H3K27me3 and Engrailed ChIP-seq data in Drosophila third instar larval brains and disks

Project description:Genome-wide binding profile of PcG proteins Pho and Ph, H3K27me3 and Engrailed in Drosophila third instar larval brains and disks, in duplicate, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed. Drosophila third instar larval brains and disks were dissected and fixed with 2% HCHO before sonication. After sonication, chromatin immunoprecipitation using anti-Pho, -Ph, -H3K27me3, and -Engrailed antibodies was carried out.

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Pho and Ph, H3K27me3 and Engrailed ChIP-seq data in Drosophila third instar larval brains and disks

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4C-seq data and Pho and Ph, H3K27me3 and Engrailed ChIP-seq data in wildtype and PRE-deleted third instar Drosophila larval brains and disks

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Pho and Ph, H3K27me3 and Cg ChIP-seq data in Drosophila third instar larval brains and discs

Project description:Genome-wide binding profile of PcG proteins- Ph, H3K27me3 and Cg in Drosophila third instar larval brains and disks, in duplicate, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed. Drosophila third instar larval brains and discs were dissected and fixed with 2% HCHO before sonication. After sonication, chromatin immunoprecipitation was carried out.

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4C-seq data and Pho and Ph, H3K27me3 and Engrailed ChIP-seq data in wildtype and PRE-deleted third instar Drosophila larval brains and disks

Project description:This SuperSeries is composed of the SubSeries listed below.

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4C-seq data and Pho and Ph, H3K27me3 and Engrailed ChIP-seq data in wildtype and PRE-deleted third instar Drosophila larval brains and disks

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Stenotrophomonas sp. pho isolate:pho

Project description:Stenotrophomonas sp. pho isolate:pho Genome sequencing

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Context dependent role of Pho binding sites in Polycomb complex recruitment in Drosophila

Project description:Polycomb group (PcG) proteins maintain the silenced state of key developmental genes, but how these proteins are recruited to specific regions of the genome is still not completely understood. In Drosophila, PcG proteins are recruited to Polycomb response elements (PREs) comprised of a flexible array of sites for sequence-specific DNA binding proteins, “PcG recruiters”, including Pho, Spps, Cg, GAF and many others. Pho is thought to play a central role in PcG recruitment. Early data showed that mutation of Pho binding sites in PREs in transgenes abrogated the ability of those PREs to repress gene expression. In contrast, genome-wide experiments in pho mutants or by Pho knockdown showed that PcG proteins can bind to PREs in the absence of Pho. Here we directly addressed the importance of Pho binding sites in two engrailed (en) PREs at the endogenous locus and in transgenes. Our results show that Pho binding sites are required for PRE activity in transgenes with a single PRE. In a transgene, two PREs together lead to stronger, more stable repression and confer some resistance to the loss of Pho binding sites. Making the same mutation in Pho binding sites has little effect on PcG-protein binding at the endogenous en gene. Overall, our data support the model that Pho is important for PcG binding but emphasize how multiple PREs and chromatin environment increase the ability of PREs to function in the absence of Pho.

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