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Identification of heat stress-responsive rice microRNAs and their target genes by high-throughput sequencing and degradome analysis

ABSTRACT: In this study, we identified a set of heat stress-responsive miRNAs and verified their targets through degradome sequencing. Nine libraries (triplicate for 0 h, 12 h and 24 h heat treatment) were constructed and sequenced. Four hundred and nineteen conserved miRNAs and 77 novel miRNAs were identified, of which, 40 conserved and two novel miRNAs were expressed differentially. Quantitative PCR of three selected miRNAs and their targets confirmed that these loci were responsive to heat stress. A total of 1364 target transcripts were identified for 114 conserved miRNA and a total of 65 were identified for 22 novel miRNA families. Gene function and pathway analyses showed that these genes probably play vital roles in rice heat stress tolerance.

ORGANISM(S): Oryza sativa Indica Group

PROVIDER: GSE77510 | GEO | 2017/12/21

SECONDARY ACCESSION(S): PRJNA310656

REPOSITORIES: GEO

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