Dataset Information

Deep Sequencing Leads to the Identification of Eukaryotic Translation Initiation Factor 5A as a Key Element in Rsv1-Mediated Lethal Systemic Hypersensitive Response to Soybean Mosaic Virus Infection in Soybean

ABSTRACT: We reported a genome-wide investigation to gain insight into the molecular signaling pathway involved in SMV G7 provoking Rsv1-mediated LSHR based on sRNA-seq, degradome-seq and transcriptome-seq analysis.

ORGANISM(S): Glycine max

PROVIDER: GSE77796 | GEO | 2016/12/31

SECONDARY ACCESSION(S): PRJNA311571

REPOSITORIES: GEO

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Project description:Resistance (R) genes are very effective for disease control in plants. Current research shows that R genes predominantly function by inducing a hypersensitive reaction (HR), which results in localized cell death thought to restrict pathogen spread. Some R genes elicit a more atypical response where resistance is not associated with HR or the associated gene expression changes, termed extreme resistance (ER). The molecular regulatory mechanism underlying ER is largely unexplored. One of the few known, naturally occurring, instances of ER is resistance derived from the soybean Rsv3 gene, which confers resistance against the most virulent strains of soybean mosaic virus (SMV). To discern the regulatory mechanism underlying the Rsv3-mediated ER reaction, we generated a gene regulatory network using transcriptomic data from a time course comparison of SMV-G7-infected resistant (L29, Rsv3-genotype) and susceptible (Williams82, rsv3-genotype) soybean cultivars. We identified putative interactions between transcription factors (TFs) regulating gene expression in hormone regulatory pathways, such as abscisic acid (ABA) and jasmonic acid (JA). This is consistent with the demonstrated involvement of these pathways in Rsv3-mediated resistance. We found significant enrichment for the G-box motif (“CACGTG”) among genes implicated in ABA- and JA-related activities. This motif is specifically recognized by MYC2, which is a master regulator of ABA and JA signaling. Our network identified a MYC2 TF encoded by Glyma.07G051500 as a putative transcriptional regulator whose expression was significantly down-regulated in L29. This correlated with the down-regulation of expression of genes involved in ABA and JA processes. Our results suggest an important function for Glyma.07G051500 in ABA and JA derived defense signaling. Additionally, our regulatory network found other putative TFs with differential expression, such as MYBs and ERFs, which may also be involved in regulating ABA and JA signaling for defense. The regulatory network presented here offers new insights into the regulation of the molecular defense mechanism underlying Rsv3-mediated ER against SMV.

Dataset Information

Deep Sequencing Leads to the Identification of Eukaryotic Translation Initiation Factor 5A as a Key Element in Rsv1-Mediated Lethal Systemic Hypersensitive Response to Soybean Mosaic Virus Infection in Soybean

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