Genomics

Dataset Information

36

Differential gene expression of T-cell lymphomas (TCL) upon engagement of T-cell receptor (TCR) signaling [TCL1; T8ML1]


ABSTRACT: To study differential gene expression of TCLs upon TCR signaling, three TCL primary cells and one TCL cell line T8ML1 were chosen for this study. The prmiary TCL cells consist of TCL1 and TCL2 (sezary patients) and TCL3 (PTCL, NOS patient). The TCR signaling was engaged by anti-CD3/CD28 treatment in vitro. The TCL cells were treated without/with anti-CD3/CD28 for different time periods in vitro in cell culture. The total RNA was isolated from the TCLs and subjected to affimetry microarray (GPL17692) analysis. The differential gene expression of individual TCL was identified as well as a set of common genes invloved in TCR signaling in TCLs. The subject for this study includes pirmary cells isolated from three TCL patients [two sezary patients (TCL1 & TCL2) and one PTCL, NOS patient (TCL3)] and cells from a TCL cell line (T8ML1). The purity of isolated primary cells is >93% as idnetified by flow cytometry. The TCLs were treated with/without anti-CD3/CD28 for different time points, with 2-3 replicates for each time point. Gene expression value was presented as Log2 transformed. Differntial expressed genes were identified by ∆log2x>1 in comparison to no anti-CD3/CD28 treatment. Overall design: TCR signaling of TCLs was stimulated by anti-CD3/CD28 for vairous time points, with triplicates for each time points. The total RNAs were extracted and converted to cDNA for microarray analysis.

INSTRUMENT(S): [HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version]

SUBMITTER: Ryan A Wilcox  

PROVIDER: GSE77875 | GEO | 2017-02-10

SECONDARY ACCESSION(S): PRJNA311941

REPOSITORIES: GEO

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Publications

T-cell Receptor Signaling Activates an ITK/NF-κB/GATA-3 axis in T-cell Lymphomas Facilitating Resistance to Chemotherapy.

Wang Tianjiao T   Lu Ye Y   Polk Avery A   Chowdhury Pinki P   Murga-Zamalloa Carlos C   Fujiwara Hiroshi H   Suemori Koichiro K   Beyersdorf Niklas N   Hristov Alexandra C AC   Lim Megan S MS   Bailey Nathanael G NG   Wilcox Ryan A RA  

Clinical cancer research : an official journal of the American Association for Cancer Research 20161025 10


<b>Purpose:</b> T-cell lymphomas are a molecularly heterogeneous group of non-Hodgkin lymphomas (NHL) that account for a disproportionate number of NHL disease-related deaths due to their inherent and acquired resistance to standard multiagent chemotherapy regimens. Despite their molecular heterogeneity and frequent loss of various T cell-specific receptors, the T-cell antigen receptor is retained in the majority of these lymphomas. As T-cell receptor (TCR) engagement activates a number of signa  ...[more]

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