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SATB2 induces transcriptional programs in melanoma that lead to metastatic behavior [RNA-Seq]

ABSTRACT: We report gene expression data for zebrafish melanomas overexpressing human SATB2 and EGFP.

ORGANISM(S): Danio rerio

PROVIDER: GSE77922 | GEO | 2021/02/08

REPOSITORIES: GEO

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SATB2 induces transcriptional programs in melanoma that lead to metastatic behavior [ChIP-Seq]

Project description:We report the distribution of human SATB2 in a primary zebrafish melanoma that overexpresses human SATB2, and H3K27Ac histone marks in zebrafish melanomas overexpressing either EGFP (control) or human SATB2.

2021-02-08 | GSE77921 | GEO

SATB2 induces transcriptional programs in melanoma that lead to metastatic behavior

Project description:SATB2 induces transcriptional programs in melanoma that lead to metastatic behavior

| PRJNA312106 | ENA

SATB2 induces transcriptional programs in melanoma that lead to metastatic behavior

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-02-08 | GSE77923 | GEO

Role of Satb1 and Satb2 in embryonic stem cells

Project description:Satb1 and Satb2 are regulators of higher order chromatin in T cells and osteoblasts repectively. We were interested if Satb1 and Satb2 play a role in the regulation of gene expression in ES cells. This SuperSeries is composed of the following subset Series: GSE17487: Expression data in WT and Satb1-/- ES cells GSE17488: Expression data in WT and ES cells overexpressing Satb1 GSE17489: Expression data in WT and ES cells overexpressing Satb2 Refer to individual Series

2009-09-01 | E-GEOD-17490 | biostudies-arrayexpress

ChIP-seq for Satb2 in E15 cortex

Project description:The goal of the study was to identify the binding site of SATB2 in wild-ype cortex by performing ChIP-seq using SATB2 antibody. E15 cortical tissues were dissected, lysed and fixed. Chromatin was prepared by sonication. Sequences bound by SATB2 protein was precipitated using a SATB2 antibody. Sequencing was performed on Illumina Genome Analyzer II. SATB2 binding peaks were called using MACS. ChIP for Satb2, followed by sequencing on Illumina Genome Analyzer II platform

2015-09-08 | E-GEOD-68910 | biostudies-arrayexpress

ChIP-seq for Satb2 in E15 cortex

2015-09-08 | GSE68910 | GEO

RNA-seq of postnatal day 0 (P0) wild-type and Satb2-/- cortices

Project description:The goal of the study was to compare gene expression of P0 wild-type and P0 Satb2-/- cortices. Total RNAs were isolated from P0 cortices dissected from wild-type and Satb2-/- mice (n=3 for each genotype), following Qiagen RNAeasy kit instruction.Sequence libraries were made following Illumina RNA TruSeq library preparation guide.The libaries were pair-end sequenced (50nt per end). Differentially expressed genes were identified by DESEQ. Total RNAs were isolated from P0 cortices (3 control and 3 mutants), and sequenced on Illumina Genome Analyzer

2015-09-08 | E-GEOD-68911 | biostudies-arrayexpress

Expression data in WT and ES cells overexpressing Satb2

Project description:Satb1 and Satb2 are regulators of higher order chromatin in T cells and osteoblasts repectively. We were interested if Satb1 and Satb2 play a role in the regulation of gene expression in ES cells. ES cells which can inducibly express Satb1 or Satb2 were generated using the tet-ON system. In this experiment, we compared gene expression in uninduced cells to that in cells which had been induced for 24h to express Satb2 ectopcially at very high levels.

2009-09-01 | GSE17489 | GEO

Gene expression signatures of SATB2-defficient vs wild-type adult neocortex

Project description:During CNS development, the nuclear protein SATB2 is expressed in superficial cortical layers and determines projection neuron identity. In the adult CNS, SATB2 is expressed in pyramidal neurons of all cortical layers and is a regulator of synaptic plasticity and long-term memory. Common variation in SATB2 locus confers risk of schizophrenia whereas rare, de novo structural and single nucleotide variants cause severe intellectual disability and absent or limited speech. To which extent symptoms in SATB2-related human pathologies depend on developmental or adult functions of the protein remains to be established. To characterize differences in SATB2 molecular function in developing vs adult neocortex, we compared SATB2 protein interactomes and SATB2-driven gene expression programs at the two ontogenetic stages by co-IP mass spectrometry and RNAseq analyses, respectively. Our results demonstrated that 1) SATB2 interacts with different protein networks at the two ontogenetic stages, with a switch from transcriptional repression towards organization of chromatin structure and 2) SATB2 determines differential transcriptional programs in neonatal vs adult cortex.

2019-02-19 | GSE123992 | GEO

Bulk RNA-seq of CRISPR SATB2 Knockout Human Colonic Organoids

Project description:To evaluate SATB2 function in human colonic epithelium, we knocked out the SATB2 gene in 3D cultured human colonic organoid.

2021-10-05 | GSE167285 | GEO