Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human alveolar epithelial cells (A549) exposed to cigarette smoke extract

ABSTRACT: Human alveolar epithelial cells were exposed to cigarette smoke extract (CSE) for 1, 3 and 5 weeks at 1%, 5% and 10%, and gene expression was evaluated by complete transcriptome microarrays. In this study we explored the effect of cigarette smoke on the gene expression profile.

ORGANISM(S): Homo sapiens

PROVIDER: GSE77942 | GEO | 2016/02/16

SECONDARY ACCESSION(S): PRJNA312174

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Human alveolar epithelial cells (A549) exposed to cigarette smoke extract

Project description:Human alveolar epithelial cells were exposed to cigarette smoke extract (CSE) for 1, 3 and 5 weeks at 1%, 5% and 10%, and gene expression was evaluated by complete transcriptome microarrays. In this study we explored the effect of cigarette smoke on the gene expression profile. Human alveolar epithelial cells stimulated with three different concentractions of CSE (1%, 5% and 10%) and for 1, 3 and 5 weeks were used for RNA extraction and hybridization on Affymetrix microarrays.

2016-02-16 | E-GEOD-77942 | biostudies-arrayexpress

Cigarette smoke extract-treated alveolar macrophage-derived extracellular vesicles

Project description:This project is based upon the fundamental observation that alveolar macrophage-derived extracellular vesicles (AM-EVs), when internalized by neighboring epithelial cells, inhibit their infection by influenza virus. This inhibitory activity of AM-EVs is abolished when AMs are treated with cigarette smoke extract (CSE). We chose to survey the AM-EV proteome in an effort to identify candidate proteins whose abundance within EVs was downregulated by CSE treatment of AMs, thus explaining the ability of CSE to abrogate the inhibitory activity against influenza.

2020-06-09 | PXD019528 | Pride

Human primary alveolar epithelial cells (hAEC) exposed to cigarette smoke extract

Project description:hAEC cells were exposed to cigarette smoke extract (CSE) or PBS for 48h, and gene expression was evaluated by RNA-seq.In this study we explored the effect of cigarette smoke on the gene expression profile.

2021-10-28 | GSE186359 | GEO

A549 cigarette smoke resistant cell line expression profiling

Project description:Chronic obstructive pulmonary disease (COPD) is currently the third cause of death worldwide with still increasing mortality and morbidity. Primary etiology of COPD is cigarette smoking. However, in clinic, not all smokers develop COPD. The underlying mechanism remains unclear. A549 cells, which are widely used in vitro as a model of alveolar type II pulmonary epithelium, were subjected to step-wise increasing cigarette smoke extract (CSE) treatments. Those cigarette smoke extract resistant (SER) cells were cultured and used for further experiments. The aim of this study is to investigate the differentially expressed genes in SER group with or without CSE treatment and identify potential genes or pathways which could play a role in COPD pathogenesis.

2024-01-01 | GSE222965 | GEO

Human airway epithelial responses to rhinovirus infection and cigarette smoke extract alone and in combination

2011-03-15 | GSE27973 | GEO

Human airway epithelial responses to rhinovirus infection and cigarette smoke extract alone and in combination

Project description:This study was performed to test the hypothesis that cigarette smoke extract would alter the responses of primary cultures of human bronchial epithelial cells to infection with purified human rhinovirus 16. The data show marked alterations in rhinovirus-induced expression profiles of a number of genes in the presence of cigarette smoke extract (CSE). Cultured epithelial cells from each of 4 donors were exposed to medium alone, rhinovirus 16 (RV16) alone, CSE alone, or RV16 in the presence of CSE. After a 24 h incubation gene expression was assessed.

2011-03-15 | E-GEOD-27973 | biostudies-arrayexpress

Cigarette smoke-induced ferritinophagy exacerbates BMSCs and skeletal development

Project description:To investigate the underlying mechanism of damage to bone and bone marrow mesenchymal stem cells stimulated by cigarette smoke extract (CSE)

2023-10-25 | GSE206079 | GEO

Whole transcriptome analysis of human kidney cells after exposure to cigarette smoke extract.

Project description:Cigarette smoke (CS) is one of risk factor to chronic obstructive pulmonary disease that is the major causes of death in the world. Furthermore, CS is an independent risk factor for chronic kidney disease (CKD) in the general adult population. The goal of this project was to identified the mechanisms of renal damage that might be associated with exposure to CS extract (CSE) in human kidney proximal tubular epithelial cell line (HK-2 cells) cells.

2022-03-16 | GSE182541 | GEO

Mitochondrial mutations in cigarette smoke extract-chronically treated human oral OKF6 cells

Project description:The objective of this study was to analyze the mitochondrial mutations induced by chronic cigarette smoke extract treatment in human oral immortal OKF6 cells. The objective of this study was to analyze the mitochondrial mutations induced by chronic cigarette smoke extract treatment in human oral immortal OKF6 cells.

2011-02-10 | GSE24414 | GEO

Human primary alveolar epithelial cells (hAEC) exposed to cigarette smoke extract

Project description:Human primary alveolar epithelial cells (hAEC) exposed to cigarette smoke extract

| PRJNA773500 | ENA