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Whole transcriptome profiling of Human Hepatoma culture Huh7.5 and Huh7.5M

ABSTRACT: Huh7.5 cells, when grown in presence of 1-2mg/ml of Geneticin, generarated colonies with cells that were morphologically distinct from the parental cells. These cells were substantially smaller in size, had spindle like features and grew faster. They expressed mesenchymal markers and were named as Huh7.5M cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE78085 | GEO | 2017/02/01

SECONDARY ACCESSION(S): PRJNA312583

REPOSITORIES: GEO

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Whole translatome profiling of Human Hepatoma culture Huh7.5 and Huh7.5M

Project description:Huh7.5 cells, when grown in presence of 1-2mg/ml of Geneticin, generarated colonies with cells that were morphologically distinct from the parental cells. These cells were substantially smaller in size, had spindle like features and grew faster. They expressed mesenchymal markers and were named as Huh7.5M cells.

2017-02-01 | GSE79464 | GEO

Control Huh7.5 cell vs HPI cell (HCV-persistently-infected Huh7.5 cell)

Project description:Transcriptional profiling of human hepatoma cell lines comparing control uninfected Huh7.5 cells with Huh7.5 cells persistently infected with HCV (HPI cell). The latter maintains and produces HCV.

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Culturing cells in human serum: differentiation and metabolic reprogramming in human hepatoma cells

Project description:Huh7.5 cells, which are cultured in human serum containing media instead of FBS containing media, undergo growth arrest and become differentiated. In this study we further characterized human serum cultured Huh7.5 cells, particularly with regards to morphology, metabolic profile and hepatocyte specific functions. Similar to primary hepatocytes, HS-cultured Huh7.5 cells become columnar, are polarized, and develop bile canalicular structures on the apical side of the cells. Further analysis by microarray showed that significant transcriptional changes occur in 22-32% of the genes. HS cultured Huh7.5 cells undergo extensive metabolic re-programming. We showed a reversal of a Warburg-like metabolic profile that is typical for proliferating cells. In HS cultured cells large lipid droplets can be found in addition to large glycogen stores. Metabolic modeling shows an increased reliance on beta-oxidation, which was confirmed by an increase in ketone body production, the end products of β-oxidation. Additionally we showed increased conversion of glucose to glycogen. Bile secretion is increased, as are the enzymes involved in the degradation of xenobiotics. Overall these data show that only changing the serum in cell culture media can result in the establishment of a cell type that morphologically, metabolically and functionally resemble primary hepatocytes.

2017-10-01 | GSE87684 | GEO

Polysome profiling of Huh7.5 cells expressing HCV NS5A

Project description:Hepatitis C Virus protein NS5A was found to upregulate assembly of cap binding initiation complex eIF4F in Huh7.5 cells. NS5A also was found to associate with translation machinery. To understand consequences of NS5A mediation in host translation, we analyzed mRNA associated with polysome fractions of NS5A expressing Huh7.5 cells and compared them with the corresponding fractions from control cells.

2014-05-01 | GSE46273 | GEO

Polysome profiling of Huh7.5 cells expressing HCV NS5A

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Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-7 mimic

Project description:Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-7 mimic

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Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-124 mimic

Project description:Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-124 mimic

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Homo sapiens isolate:Huh7.5

Project description:Homo sapiens isolate:Huh7.5 Raw sequence reads

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Exosome-mediated transmission of hepatitis C virus between human hepatoma Huh7.5 cells.

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Profile of gene expression with HCV infection in Huh7.5 cells

Project description:Differential expression study of lncRNAs (long non-coding RNAs) and mRNAs in HCV infection. For this we infected Huh7.5 cells with HCV (JFH-1) virus and control cells were mock infected. 48h post infection we isolated total RNA using qiagen RNeasy kit as per manufacturer's protocol. The total RNA was then subjected to microarray for both lncRNAs and mRNAs. The resultant profile can be used to compare gene expression in HCV infection versus mock infected cells.

2016-05-01 | GSE76333 | GEO

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