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Hela cell line microRNA profiles following CFIm25 knockdown

ABSTRACT: Purpose: When CFIm25 knockdown induces global APA events, we aim to investigate ceRNA landscape change based on microRNA expression change. Methods: microRNA from HeLa cells treated with control siRNA and CFIm25 siRNA were subject to RNA-Seq. Results: Consistent with our observations in TCGA breast cancer, we found a surprisingly high enrichment of 3ʹUTR shortening genes' ceRNA partners to tumor suppressors and their down-regulation. Conclusion: Our work indicated that the shortened 3ʹ-UTRs might direct the released miRNAs to repress their ceRNA partners in trans, which are enriched in ceRNET hubs and tumor suppressors, thereby effectively disrupting normal ceRNET and contributing to tumorigenesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE78198 | GEO | 2018/02/20

REPOSITORIES: GEO

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CFIm25 links alternative polyadenylation to glioblastoma tumour suppression

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