CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

ABSTRACT: Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with three-fold higher sensitivity, lower costs, and less hands-on time. We also implemented CEL-Seq2 on Fluidigm’s C1 system, thereby providing its first single-cell, on-chip barcoding method, and detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2’s increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use

OTHER RELATED OMICS DATASETS IN: PRJNA390541 PRJNA313513

ORGANISM(S): Caenorhabditis elegans Mus musculus

PROVIDER: GSE78779 | GEO | 2016/04/08

SECONDARY ACCESSION(S): PRJNA313513

REPOSITORIES: GEO