Effects of RPAP1 depletion on mRNA gene expression (RNA-seq) and RNA Pol II abundance (ChIP-seq) in primary mouse embryonic fibroblasts or embryonic stem cells.

ABSTRACT: Here, we investigated the role of RPAP1 in mammalian cell identity. As in plants, interfering with RPAP1 function did not affect the viability of mouse embryonic stem cells, but severely impaired their differentiation capacity (RNA-Seq#2 data, below). Conversely, in somatic cells, RPAP1 was essential for the expression of lineage specifying factors and viability (RNA-seq#1 data, below). Moreover, inhibition of RPAP1 triggers dedifferentiation and facilitates reprogramming into pluripotent stem cells. Mechanistically, RPAP1 maintains transcribing Pol II levels and Pol II Ser5 phosphorylation, particularly on developmental genes (ChIP-seq data, below).

ORGANISM(S): Mus musculus

PROVIDER: GSE78795 | GEO | 2017/11/24

SECONDARY ACCESSION(S): PRJNA313941

REPOSITORIES: GEO