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The anti-tumor core molecular targets of TSA in cholangiocarcinoma

ABSTRACT: Firstly our study demonstrated that TSA can inhibits cell proliferation, induces cell apoptosis and cell cycle arrest in CCA cell lines in vitro. To identify the target transcripts of TSA to suppress CCA tumorigenesis, mRNA expression profiles were determined by microarray analysis. We chose TFK-1 cells treated by TSA in indicated concentration (IC50) 48 hours for the microarray. Then we found out TACC3 was downregulated, and demonstrated that TACC3 was high expression and may played a role as an target of TSA in inhibiting CCA cells proliferation and migration via in vitro and vivo experiments.

ORGANISM(S): Homo sapiens

PROVIDER: GSE78867 | GEO | 2016/03/15

SECONDARY ACCESSION(S): PRJNA314227

REPOSITORIES: GEO

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The anti-tumor core molecular targets of TSA in cholangiocarcinoma

Project description:Firstly our study demonstrated that TSA can inhibits cell proliferation, induces cell apoptosis and cell cycle arrest in CCA cell lines in vitro. To identify the target transcripts of TSA to suppress CCA tumorigenesis, mRNA expression profiles were determined by microarray analysis. We chose TFK-1 cells treated by TSA in indicated concentration (IC50) 48 hours for the microarray. Then we found out TACC3 was downregulated, and demonstrated that TACC3 was high expression and may played a role as an target of TSA in inhibiting CCA cells proliferation and migration via in vitro and vivo experiments. We chose TFK-1 cells treated by TSA in indicated concentration (IC50) 48 hours as the experiment sample and without TSA as the control sample to do this microarray analysis for three times. Then regulated genes would be tested to investigate the potential roles in CCA tumorigenesis,

2016-03-15 | E-GEOD-78867 | biostudies-arrayexpress

TSA-DC Vaccine in Treating Patients With Gastrointestinal Solid Tumor

Project description:The goal of this study is to learn about the safety and tolerance of autologous TSA-DC cell and evaluate the efficacy and feasibility of the cell therapy compared to the patients’ past standard regimen. 20 gastrointestinal solid tumors subjects failed from at least one systemic therapy will be enrolled into the trial and receive a succession of treatment of TSA-DC vaccine.

| 2246857 | ecrin-mdr-crc

Gene expression analysis of RNAs isolated from TSA-untreated and TSA-treated human keratinocytes

Project description:In order to identify the TSA responsive genes, we performed a gene expression microarray analysis for the RNAs isolated from TSA-untreated and TSA-treated human keratinocytes

2018-12-31 | GSE121902 | GEO

mRNA and lncRNA expression in cholangiocarcinoma

Project description:Long noncoding RNAs (lncRNAs) are known to play important roles in different cell contexts, including cancers. However, little is known about lncRNAs in cholangiocarcinoma (CCA), a cholangiocyte malignancy with poor prognosis, associated with chronic inflammation and damage to the biliary epithelium. This study determined whether lncRNAs were dysregulated and participated in disease diagnosis or pivotal inflammation pathways through a genome-wide lncRNA screening and functional analysis. We firstly identified a large number of lncRNAs abnormally expressed between 9 pairs of cancerous and adjacent tissues of CCA, and between intra-hepatic CCA and extra-hepatic CCA through a genome-wide profiling. Our findings show that lncRNAs may be potential biomarkers of CCA progression.

2017-09-16 | GSE103909 | GEO

Mixed hepatocellular – cholangiocarcinoma derives from liver progenitor cells and depends on senescence and IL6 trans-signaling, bulk RNA sequencing

Project description:Mixed hepatocellular – cholangiocarcinoma (HCC-CCA) is a highly malignant primary liver cancer of increasing incidence for which the cell of origin and molecular pathogenesis have not yet been fully elucidated. Here, by linking YFP to the progenitor cell specific promoter of Foxl1 in Mdr2-KO mice, we generated a lineage tracing mouse model for liver cancer to monitor the development of mixed HCC-CCA tumors following a prolonged period of liver inflammation. In this model of chronic hepatitis, we demonstrate that liver progenitor cells are the source of mixed HCC-CCA tumors but not of hepatocellular carcinoma (HCC). Moreover, we show that IL6, originating from senescent hepatic cells, is involved in progenitor cell proliferation and development of mixed HCC-CCA tumors via IL6 trans-signaling. These findings could form the basis for the development of novel therapeutic approaches for treatment of mixed HCC-CCA liver cancer.

2023-11-15 | GSE201995 | GEO

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Mixed hepatocellular – cholangiocarcinoma derives from liver progenitor cells and depends on senescence and IL6 trans-signaling, single-cell RNA sequencing

2023-11-15 | GSE202089 | GEO

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Next generation sequencing of human cholangiocarcinoma cells and associated stem-like component

Project description:The goal of this study is to provide a global transcriptome profiling (RNA-seq) of stem-like subset in human cholangiocarcinoma (CCA). Functional enrichment of CCA stem-like subset was performed by 3D sphere culture (SPH) in CCA cell lines. Comparison to parental CCA cells grown as 2D monolayer is provided.

2022-06-01 | GSE162845 | GEO

Patient-derived model combining organoids and matrix reveals cholangiocarcinoma promoting properties of the microenvironment

Project description:Cholangiocarcinoma (CCA) is a rare but aggressive type of primary liver cancer, with an overall 5-year survival rate of <10%. The extracellular matrix (ECM) plays a particularly important role in CCA tumor biology, manifested in the desmoplastic tumor environment. This study aim is to design an in vitro CCA model combining patient-derived cells and patient-derived ECM mimicking the native microenvironment. decellularized Patient CCA tissue (CCA-matrix; CCA-M) and tumor-free liver tissue (TFL-M) was decellularized and repopulated with CCA organoids (CCAOs). Culture was performed with amino acids labeled with stable heavy isotopes to enable separation of pre-existing ECM from proteins produced by the cells in the mass spectrometry (MS) analysis. CCAOs on decellularized matrix form complex morphological structures, with environment-dependent proliferation and migration dynamics, driven by the occurrence of EMT. CCAOs in TFL-M or CCA-M can be used to study the initiation or progression of desmoplasia in CCA, respectively. Furthermore, in-depth transcriptomic and proteomic analysis reveals that CCA-M induces a transcriptome more resembling in vivo CCA tumor tissue, with an accompanying protective effect of the desmoplastic environment on tumor survival.

2023-03-04 | PXD028408 | Pride

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