Project description:H3K4me1 binding in murine pre-B cells detected by ChIP-seq. For the ChIP-seq, input and immunoprecipitated DNA was given to the TSRI Next Generation Sequencing Core (the Scripps Research Institute, La Jolla, CA, US), where it was prepared for massively parallel sequencing on Illumina HiSeq2000.
Project description:Here we used ChIP-MS to quantitatively profile chromatin-associated proteins that are specifically associated with H3K4me1- and H3K4me3-modified nucleosomes in IMR-90 chromatin.
Project description:Histone modifcations and CTCF binding at the c-myb locus were compared in cell lines with c-myb expressing, which are myeloblatic M1 cells and leukemia cells with virus integration, VS. M1 cells without c-myb expression induced by IL-6. Distribution of active histone marks at the c-myb gene and the upstream regions are associated with active c-myb transcription. The enrichment of all of these active histone marks decreased with differentiation-induced down-regulation of c-myb, but increased and spread in tumor cells. ChIP-on-chip from murine myeloid cell line M1 and virus-induced myeloid leukemia cell lines for H3K4me3, H3K9/14ac, H3K4me1, H3K27me3, H3K9me3 and CTCF
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays H3K4me1 at 12 different time-points of Drosophila development. Current Dataset: [GSM386042..GSM386050]: ChIP-chip of H3K4Me1 in Drosophila embryos at 4-8 hours of development [GSM386051..GSM386059]: ChIP-chip of H3K4me1 in Drosophila embryos at 12-16 hours of development [GSM386060..GSM386068]: ChIP-chip of H3K4me1 in Drosophila embryos at 16-20 hours of development [GSM386069..GSM386077]: ChIP-chip of H3K4me1 in Drosophila embryos at 20-24 hours of development [GSM418275..GSM418283]: ChIP-chip of H3K4me1 in Drosophila embryos at 8-12 hours of development [GSM442224..GSM442232]: ChIP-chip of H3K4me1 in Drosophila embryos at 0-4 hours of development [GSM442233..GSM442241]: ChIP-chip of H3K4me1 in Drosophila Pupae [GSM442242..GSM442250]: ChIP-chip of H3K4me1 in Drosophila L3 [GSM442251..GSM442259]: ChIP-chip of H3K4me1 in Drosophila L2 [GSM442260..GSM442268]: ChIP-chip of H3K4me1 in Drosophila L1 [GSM442269..GSM442277]: ChIP-chip of H3K4me1 in Drosophila Adult male [GSM442278..GSM442286]: ChIP-chip of H3K4me1 in Drosophila Adulat female For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Project description:Monomethylation of histone H3 lysine 4 (H3K4me1) marks enhancers in mammals. However, the function of H3K4me1 in plants remains largely unclear. Here, we analyzed the genome-wide distribution of H3K4me1 in diverse species across evolution, revealing a distinctive H3K4me1 distribution pattern in land plants. To explore the function of H3K4me1 in plants, we identified an H3K4me1-specific reader protein, Early heading date 3 (Ehd3), and solved the structure of Ehd3 in complex with the H3K4me1 peptide, revealing a unique binding module differing from the previously reported PHD finger proteins. We further identified an Ehd3-binding protein, SET domain group 724 (SDG724), and the deletion of either Ehd3 or SDG724 caused similar defects in plant phenotype and changes in transcriptome and epigenome profiles. Both Ehd3 and SDG724 are enriched at chromatin regions marked by H3K4me1 but not H3K4me2 or H3K4me3. Ehd3 activates the H3K36 methyltransferase SDG724, and H3K36me2/me3 are colocalized with H3K4me1 in the genomes of land plants. Collectively, our results reveal that H3K4me1 directs the establishment of H3K36me2 and H3K36me3 in land plants.
Project description:ChIP-seq analysis of AR, Sp1, H3K27Ac, and H3K4me1; The genime-wide distribution of AR, Sp1, H3K27Ac, and H3K4me1 druing murine embryonic external genitalia (eExG) sex differentiation was shown.