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The midguts transcriptome of Aedes aegypti (Liverpool strain) females knock down AaMesh and feed AaMesh antibody versus those knock down GFP and feed pre-immune.

ABSTRACT: We reported the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti midguts knock down 3, 6days, feed antibody 18h transcriptome.

ORGANISM(S): Aedes aegypti

PROVIDER: GSE79070 | GEO | 2016/03/11

SECONDARY ACCESSION(S): PRJNA314871

REPOSITORIES: GEO

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The midguts transcriptome of Aedes aegypti (Liverpool strain) females knock down AaMesh and feed AaMesh antibody versus those knock down GFP and feed pre-immune.

Project description:We reported the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti midguts knock down 3, 6days, feed antibody 18h transcriptome. Comparison of the midguts transcriptome of Aedes aegypti females at two knockdown time points and one feed condition; GFP dsRNA-3 or -6days: 3 or 6 days after 7day-old mosquitos were microinjected GFP dsRNA AaMesh dsRNA-3 or -6days: 3 or 6 days after 7day-old mosquitos were microinjected AaMesh dsRNA Pre-immune-18h: 18hrs after 7day-old wild type mosquitos were fed with Pre-immune AaMesh antibody-18h: 18hrs after 7day-old wild type mosquitos were fed with AaMesh antibody

2016-03-11 | E-GEOD-79070 | biostudies-arrayexpress

The midguts transcriptome of Aedes aegypti (Liverpool strain) females blood fed DENV2 NS1 versus those blood fed BSA

Project description:We report the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti midguts transcriptome 4,8,18 hours after blood feeding BSA or DENV2 NS1.

2016-05-23 | GSE73967 | GEO

Relative quantitative proteomics of uninfected vs wMel infected Aedes aegypti midguts

Project description:Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important new dengue control strategy. To better understand the mechanisms of virus inhibition, proteomic quantification of the effects of Wolbachia in mosquito (Aedes aegypti) midguts was performed.

2017-09-19 | PXD006239 | Pride

siRNA sequencing of Aedes aegypti from two regions of the Americas reveals insect-specific virus profiles with geographic structure

Project description:We performed small RNA sequencing on recently colonized female Aedes aegypti from Mexico and Brazil. We compare small RNA profiles in midguts and abdomens (without ovaries) either non-bloodfed or 48 hours post non-infectious bloodmeal.

2021-07-01 | GSE154531 | GEO

Aedes aegypti sex specific gene expression

Project description:This analysis compare gene expression between 4 day old sugar fed female and male Aedes aegypti mosquitoes. Keywords: Aedes aegypti sex specific expression

2007-05-15 | GSE7813 | GEO

The transcriptome of Aedes aegypti (Liverpool strain) females kept on a sugar diet versus those blood-fed

Project description:We report the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti transcriptome 5 hours after blood feeding.

2011-03-01 | GSE24872 | GEO

Aedes aegypti

Project description:The midguts transcriptome of Aedes aegypti (Liverpool strain) females blood fed DENV2 NS1 versus those blood fed BSA

| PRJNA298592 | ENA

The transcriptome of Aedes aegypti (Liverpool strain) females kept on a sugar diet versus those blood-fed

Project description:We report the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti transcriptome 5 hours after blood feeding. Comparison of the transcriptome of Aedes aegypti females at two physiological conditions and one time point.

2011-03-01 | E-GEOD-24872 | biostudies-arrayexpress

Developmental analysis of Aedes aegypti gene expression

Project description:This analysis defines the adult female and developmental specific transcriptomes of Aedes aegypti. Keywords: Aedews aegypti, development, gene expression

2007-05-15 | GSE7811 | GEO

SAGE analysis reveals variation in midgut transcriptomes of dengue-2 susceptible and refractory Aedes aegypti mosquitoes

Project description:Oral susceptibility of Aedes aegypti mosquitoes to dengue viruses varies between different Aedes species and strains. However, the midgut-specific transcriptional profile that may produce this variation is presently obscure and was the subject of our investigation. The variation in active expression between dengue-2 susceptible (SUS) and refractory (REF) mosquitoes was investigated during the first critical 96 hours after infection Transcriptional profiles were mined from respective guts using the serial analysis of gene expression technique (SAGE) and libraries constructed from midguts obtained from mosquitoes that received a dengue-2 infected blood meal (DENV-2), a non infected blood meal (naive) or a 5% sucrose meal (SM). Here we report that variation between DENV-2 infected libraries versus respective naïve libraries revealed very few transcripts that were common and statistically significant in DENV-2 infected libraries. In addition, the expression profiles among libraries displayed up regulation of antisense transcripts especially in the SUS strain. A strong proclivity towards strain-specificity in differential expression was observed, which suggested an exclusive transcription that is likely up-regulated after DENV-2 infection

2013-09-30 | GSE14384 | GEO

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