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Gene expression profiling of PBMC from coinfected or non-coinfected mice at d0, d3, d7, and d21 post YFV-17D challenge

ABSTRACT: We tested the effects of co-infection on vaccine response to YFV-17D.

ORGANISM(S): Mus musculus

PROVIDER: GSE79466 | GEO | 2016/03/22

SECONDARY ACCESSION(S): PRJNA315889

REPOSITORIES: GEO

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Gene expression profiling of PBMC from coinfected or non-coinfected mice at d0, d3, d7, and d21 post YFV-17D challenge

Project description:We tested the effects of co-infection on vaccine response to YFV-17D. Groups of mice were divided into either Mock infected or Co-infected groups. Mock infected were housed in a biohazard facility and inoculated with PBS. Co-infected were infected sequentially with murine gammaherpesvirus-68, murine cytomegalovirus, influenza WSN, and Heligmosimoides polygyrus. Both mock and co-infected groups were challenged with Yellow Fever virus (YFV-17D). Day 0 is prior to YFV. Days 3, 7, and 21 were timepoints after YFV.

2016-03-22 | E-GEOD-79466 | biostudies-arrayexpress

ATAC-seq analysis of human CD8 T cells specific for the NS4B-214 epitope in the Yellow fever vaccine YFV-17D strain

Project description:ATAC-seq analysis of human CD8 T cells specific for the NS4B-214 epitope in the Yellow fever vaccine YFV-17D strain

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Expression data from Rhesus immune cells responding to YFV

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Expression data from Rhesus immune cells responding to YFV

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RNA-seq analysis of YFV-17D specific and total naive CD8 T cells in humans

Project description:These 14 samples originate from peripheral blood mononuclear cells (PBMC) from donors at different times after they were vaccinated with the YF-17D vaccine.

2017-12-13 | GSE100745 | GEO

Microarray analysis of total naïve and YFV-17D specific CD8 T cells in humans

Project description:CD8 T cells play an importart role in adaptive immunity to intracellular pathogens. Naïve CD8 T cells , that have not encountered antigen previously can be identified by virtue of their distinct phenotype. Upon antigenic encounter, they proliferate rapidly and undergo massive reprograming to differentiate to cytotoxic T lymphocytes. The yellow fever live virus vaccine (YF-17D) provides a model primary acute viral infection that can be used to follow this response.Here we characterize the resting, non-activated naive CD8 T cells in nine healthy adults and YF-specific CD8 T cells elicited in response to YF-17D vaccination from the same donors during the effector (2 weeks after vaccination) and memory (5-8 months later) stages.

2017-12-13 | GSE26347 | GEO

Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses (Experiment 2: scRNA-seq)

Project description:We performed an in-depth characterization and comparison of the immune system diversity and complexity in the spleen of conventional NRG-HIS mice and NFA2-HIS/Ftl3LG mice upon YFV-17D infection, a live-attenuated virus that induce that induce potent protective immunity in human. To do so, we employed Seq-Well, a recently developed platform for massively parallel single-cell RNA-Seq (scRNA-Seq), on splenocytes from NRG-HIS mice and NFA2-HIS/Flt3LG mice at six-weeks post YFV-17D infection. Our data provide an in-depth view of the cellular composition of the HIS in conventional and second-generation humanized mice. They also highlight the enhanced engraftment and functionality of the critical role of the myeloid and NK cell compartment in NFA2-HIS/Flt3LG mice, which is likely critical in promoting an enhanced transcriptomic, cellular and humoral response to YFV-17D.

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Chromatin accessibility analysis of Huh7 cells upon YFV capsid expression

Project description:ATACseq analysis of chromatin accessibility in Huh7 cells upon YFV capsid expression

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