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RNA sequencing of E11.5 Control and Prmt5cKO forelimbs

ABSTRACT: We preformed RNA sequencing on control and Prmt5cKO embryos at E11.5 to determine differentially expressed genes resulting from a loss of Prmt5.

ORGANISM(S): Mus musculus

PROVIDER: GSE79487 | GEO | 2016/12/13

SECONDARY ACCESSION(S): PRJNA315976

REPOSITORIES: GEO

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Expression profiles of E11.5 wildtype and Shox2 knockout embryonic forelimbs

Project description:The development of vertebrate extremities is a complex process which requires a highly coordinated network of different transcriptional activities. The homeodomain transcription factor Shox2 is a key player in limb formation controlling neural, muscular and skeletal development. Here, we compared gene expression profiles of wildtype and Shox2 knockout limbs using microarray experiments to identify Shox2 target genes. Forelimbs of E11.5 mouse embryos were dissected and genotyped for RNA extraction. RNA from 3-4 embryos of 2 different pregnancies was used for hybridisation to 2 arrays per genotype (wildtype and Shox2 knockout) and compared.

2014-01-16 | E-GEOD-51523 | biostudies-arrayexpress

Embryonic and adolescent forelimbs, brains, whole embryos, Aha-enrichment forelimbs, Mus musculus (wild-type, C57BlJ6/J; OIM, B6C3Fe a/a-Col1a2oim/J), proteomic dataset

Project description:Embryonic day (E)12.5 whole murine embryos, E11.5 - E14.5 whole murine embryos, E11.5 - E14.5, post-natal day (P)3 and P35 murine forelimbs, E14.5 brains, and COL1A2-mutant and COL1A2-WT forelimbs were fractionated and specific fractions were analyzed via LC-MS/MS. Aha-enrichment experiments consisted of in vivo protein labeling with azidohomoalanine (Aha) followed by tissue fractionation of the forelimbs and enrichment of labeled ECM proteins from the final IN pellet ('enriched'). 'Unenriched samples', or the background from which newly synthesized proteins were enriched from, were also analyzed via LC-MS/MS.

2020-06-05 | MSV000085556 | MassIVE

Small RNA analysis of wildtype Mouse embryo and Adar1 null mouse embryo at E11.0 and E11.5 together with mRNA-seq results of E11.5

Project description:Adar1 is an essential gene for mouse embryonic development. Adar1 null mouse embryos dies around E11.5 because of massive apoptosis. Small RNA: 4 samples examined: wild type E11.0, ADAR1 null E11.0, wild type E11.5, ADAR1 null E11.5, mRNA-seq: wild type E11.5, ADAR1 null E11.5.

2013-05-01 | E-GEOD-33473 | biostudies-arrayexpress

Small RNA analysis of wildtype Mouse embryo and Adar1 null mouse embryo at E11.0 and E11.5 together with mRNA-seq results of E11.5

Project description:Adar1 is an essential gene for mouse embryonic development. Adar1 null mouse embryos dies around E11.5 because of massive apoptosis.

2013-05-01 | GSE33473 | GEO

Transcriptomic analysis of E11.5 AGM VE cadherin populations

Project description:We sorted endothelial (EC), hematopoietic (HC) and hematopoietic stem/progenitor (HSPC) cell populations from AGM of E11.5 C57Bl6 embryos, established and compared their transcriptome to highlight specific regulators of hematopoietic emergence.

2019-08-24 | GSE133804 | GEO

Transcriptome analysis of WWC2-deficient E11.5 embryos

Project description:Transcriptome analysis of WWC2-deficient E11.5 embryos

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Pax2 Dependent Expression in the Intermediate Mesoderm at E11.5

Project description:Heterozygous and homozygous Pax2 E11.5 embryos were collected and the intermediate mesoderm was dissected and dispersed into single cells. The Pax2 positive cells also expressed EGFP, which was knocked into the Pax2 locus. EGFP positive cells were sorted by FACS and RNA isolated.

2014-12-13 | GSE64129 | GEO

DNase-seq profile of E11.5 whole mouse embryos

Project description:DNase-seq profile of E11.5 whole mouse embryos

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DNase-seq profile of E11.5 whole mouse embryos

Project description:The aim of this experiment was to profile the DNase-I accessibility landscape of E11.5 whole mouse embryos. Separate fractions were taken for DNA cleavages of length 50-100bp and 175-400bp.

2015-04-11 | E-MTAB-3468 | biostudies-arrayexpress

Expression data from E11.5 mouse branchial arch 1 (BA1) - comparison between Ezh2lox/lox and Wnt1Cre Ezh2lox/lox embryos

Project description:Conditional ablation of Ezh2 in the neural crest lineage results in loss of the neural crest-derived mesenchymal derivatives. In this data sheet we determine gene expression analysis in Ezh2lox/lox and Wnt1Cre Ezh2lox/lox in E11.5 mouse BA1 cells. Conditional ablation of Ezh2 in the neural crest lineage was achieved by crossing Wnt1creEzh2lox/wt mice with Ezh2lox/lox mouse lines. We analyzed a total of 6 samples including 3 biological replicates from Ezh2lox/lox embryos and 3 biological replicates from Wnt1cre Ezh2lox/lox embryos.

2013-11-09 | E-GEOD-52220 | biostudies-arrayexpress

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