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High throughput characterization of the m6A demethylase FTO by CLIP and RNAseq

ABSTRACT: We performed the CLIP-seq analysis of RNAs bound by 3xFLAG-tagged FTO protein stably expressed in human HEK293 cells. In parallel, we profiled the transcriptome of the same cells by RNA-seq, which was used for CLIPseq bioinformatics analysis as an input. The CRISPR-Cas9 was used to knock-out FTO expression in HEK293 to compare the mRNA expression changes and exon usage between FTO KO and WT cell lines. All experiments were performed in three replicates.

ORGANISM(S): Homo sapiens

PROVIDER: GSE79577 | GEO | 2017/08/25

SECONDARY ACCESSION(S): PRJNA316214

REPOSITORIES: GEO

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