Transcriptomics,Genomics

Dataset Information

155

Rabbit limbal epithelial side population (SP) cells and non-SP cells


ABSTRACT: Corneal epithelial stem cells reside in the limbus that is the transitional zone between the cornea and conjunctiva, and are essential to maintain the homeostasis of corneal epithelium. However, their characterization is poorly understood. Therefore, we constructed gene expression profiles of limbal epithelial SP and non-SP cell using RNA-sequencing. As a result, limbal epithelial SP cells have immature cell phenotypes with endothelial/mesenchymal cell markers, while limbal epithelial non-SP cells have epithelial progenitor cell markers. Overall design: Examination of rabbit limbal epithelial SP and non-SP cells

INSTRUMENT(S): Ion Torrent Proton (Oryctolagus cuniculus)

SUBMITTER: Sumako Kameishi 

PROVIDER: GSE79620 | GEO | 2016-04-18

SECONDARY ACCESSION(S): PRJNA316450

REPOSITORIES: GEO

altmetric image

Publications

Characterization of rabbit limbal epithelial side population cells using RNA sequencing and single-cell qRT-PCR.

Kameishi Sumako S   Umemoto Terumasa T   Matsuzaki Yu Y   Fujita Masako M   Okano Teruo T   Kato Takashi T   Yamato Masayuki M  

Biochemical and biophysical research communications 20151110 3


Corneal epithelial stem cells reside in the limbus, a transitional zone between the cornea and conjunctiva, and are essential for maintaining homeostasis in the corneal epithelium. Although our previous studies demonstrated that rabbit limbal epithelial side population (SP) cells exhibit stem cell-like phenotypes with Hoechst 33342 staining, the different characteristics and/or populations of these cells remain unclear. Therefore, in this study, we determined the gene expression profiles of limb  ...[more]

Similar Datasets

2008-04-06 | E-GEOD-10683 | ArrayExpress
| GSE10683 | GEO
2013-01-01 | E-GEOD-38190 | ArrayExpress
| GSE38190 | GEO
2010-03-24 | GSE19035 | GEO
2010-03-23 | E-GEOD-19035 | ArrayExpress
2011-06-09 | E-GEOD-29402 | ArrayExpress
2008-10-25 | E-GEOD-12604 | ArrayExpress
2008-08-29 | GSE12604 | GEO
| GSE12631 | GEO