Blood and Adipose tissue samples
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE7965 | GEO | 2007/06/13
SECONDARY ACCESSION(S): PRJNA100717
REPOSITORIES: GEO
Similar Datasets
Project description:We analyzed the expression of 23,653 transcripts in the adipose tissue of 19 individuals treated with Sibutramine Keywords: sibutramine effect 157 adipose tissue samples from 19 individuals treated with sibutramin as well as placebo were profiled for gene expression.
2010-06-24 | E-GEOD-10545 | biostudies-arrayexpress
Project description:We analyzed the expression of 23,653 transcripts in the adipose tissue of 19 individuals treated with Sibutramine Keywords: sibutramine effect
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Project description:The Greek Metabolic (GM) adipose study was carried out in 106 individuals living in Greece and consists of genotyping data (chip), RNA-Seq and ATAC-Seq (for a subset of individuals) data from paired biopsies of Subcutaneous and Visceral adipose tissue. eQTL mapping was performed to study gene regulation and findings compared to those from adipose tissue GTEX data. The focus of this project was to study an underexplored population living in different environmental conditions in order to reveal novel regulatory effects for genes that shape complex traits and disease risk. Our results highlight the utility of modest-sized studies in adding to our understanding of the molecular basis of complex traits and to the identification of mechanisms that drive disease in specific tissues.
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Project description:White adipose tissue accumulates at various sites throughout the body and some adipose tissue depots exist in close proximity to organs, whose function they could influence in a paracrine manner. Prostate gland is surrounded by a poorly characterized adipose depot called periprostatic adipose tissue (PPAT) playing emerging roles in prostate-related disorders. Unlike all other adipose depots, PPAT secretes pro-inflammatory cytokines even in lean individuals and does not increase in volume during obesity. These unique features remain unexplained due to the poor structural and functional characterization of this tissue. We characterized the structural organization of PPAT in patients in comparison to abdominopelvic adipose tissue (APAT), an extraperitoneal adipose depot whose accumulation is correlated to BMI. Unbiased comparisons of PPAT and APAT transcriptomes found that most differentially expressed genes were related to the hypoxia response. This chronic hypoxic state was associated with inflammation and fibrosis, which explain the failure of PPAT to expand in obesity and open new mechanistic avenues to explain its role in prostate-related disorders including cancer.
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Project description:Visceral adipose tissue samples were obtained from severely obese individuals that underwent bariatric surgery. The goal of this study was to identify tissue specific methylation QTLs. Whole-transcriptome subcutaneous adipose tissue methylation levels were determined in 71 individuals with a BMI >35 kg/m2.
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Project description:Subcutaneous adipose tissue samples were obtained from severely obese individuals that underwent bariatric surgery. The goal of this study was to identify tissue specific methylation QTLs. Whole-transcriptome subcutaneous adipose tissue methylation levels were determined in 71 individuals with a BMI >35 kg/m2.
2014-09-17 | GSE61450 | GEO
Project description:Visceral adipose tissue samples were obtained from severely obese individuals that underwent bariatric surgery. The goal of this study was to identify tissue specific methylation QTLs. Whole-transcriptome subcutaneous adipose tissue methylation levels were determined in 71 individuals with a BMI >35 kg/m2. Bisulphite converted DNA from the 71 visceral adipose tissue samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip.
2014-09-17 | E-GEOD-61453 | biostudies-arrayexpress
Project description:Subcutaneous adipose tissue samples were obtained from severely obese individuals that underwent bariatric surgery. The goal of this study was to identify tissue specific methylation QTLs. Whole-transcriptome subcutaneous adipose tissue methylation levels were determined in 71 individuals with a BMI >35 kg/m2. Bisulphite converted DNA from the 71 subcutaneous adipose tissue samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip.
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2014-11-14 | E-GEOD-55200 | biostudies-arrayexpress
Project description:To map the genetics of gene expression in metabolically relevant tissues and investigate the diversity of expression SNPs (eSNPs) in multiple tissues from the same individual, we collected four tissues from approximately 1,000 patients undergoing Roux-en-y gastric bypass and clinical traits associated with their weight loss and co-morbidities. We then performed high-throughput genotyping and gene expression profiling and carried out a genome-wide association analyses for more than one hundred thousand gene expression traits representing four metabolically relevant tissues; liver, subcutaneous adipose, omental adipose and stomach. We successfully identified 24,531 eSNPs corresponding to ~10,000 distinct genes. This represents the greatest number of eSNPs identified to our knowledge by any study to date and the first study to identify eSNPs from stomach tissue. We then demonstrate how these eSNPs provide a high quality disease map for each tissue in morbidly obese patients to not only inform genetic associations indentified in this cohort, but in previously published genome wide association studies as well. eSNPs and gene co-expression modules identification in morbidly obese patients represent a great resource that will aid in elucidating the key networks associated with morbid obesity, response to RYGB and disease as a whole. Keywords: Tissue profiling in a human cohort. Subcutaneous adipose tissue was collected from patients at the time of RYGB surgery at Massachusetts General Hospital between 2000 and 2007. Samples were collected in RNAlater (Ambion/Applied Biosystems), stored at -80° and shipped to Rosetta Inpharmatics Gene Expression Laboratory Seattle, WA for extraction, amplification, labeling, and microarray processing. Samples processed ranged in size from 100-200mg. Total RNA extracted from subcutaneous adipose was converted to fluorescently labeled cRNA that was hybridized to custom 44K DNA oligonucleotide microarrays manufactured by Agilent Technologies as described previously (Hughes et al. 2001; Schadt et al. 2008). Successful gene expression profiling results were collected from 701 subcutaneous adipose samples.
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