Transcriptomics,Genomics

Dataset Information

46

Gene expression data from Mycobacterium tuberculosis-infected WT and miR-155-/- bone-marrow derived macrophages [timecourse]


ABSTRACT: How the complex interaction between Mycobacterium tuberculosis (Mtb) and the host is regulated during infection is still not well understood. Using a systems biology approach, we demonstrate here that miR-155 is one of several microRNAs that regulate host gene expression over the first 48 hours of Mtb infection in macrophages. miR-155 regulates the cell survival of Mtb-infected macrophages through SHIP1/AKT signaling. Using timecourse gene expression data, we constructed a miRNA regulatory network for the innate immune response to Mtb infection by WT macrophages. The network suggested a role for seven miRNAs in regulating the host response to Mtb, with miR-155 being one of them. We then validated a role for miR-155 by comparing the response between WT and miR-155-/- macrophages. Overall design: Three replicates of WT C57BL/6 BMMs were collected at 0, 4, 8, 24, and 48 hours post-infection with Mycobacterium tuberculosis, H37Rv strain (MOI 5). The regulatory network was then validated with WT and miR-155-/- BMM samples collected at 0 and 8 hours post-infection (three replicates each). This dataset represents three replicates of WT C57BL/6 BMMs were collected at 0, 4, 8, 24, and 48 hours post-infection with Mycobacterium tuberculosis, H37Rv strain (MOI 5).

INSTRUMENT(S): [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [Alternative CDF: BrainArray version 14: MoEx10stv1_Mm_ENTREZG]

SUBMITTER: Aderem Lab  

PROVIDER: GSE79731 | GEO | 2016-09-14

SECONDARY ACCESSION(S): PRJNA316925

REPOSITORIES: GEO

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Publications

MiR-155-regulated molecular network orchestrates cell fate in the innate and adaptive immune response to Mycobacterium tuberculosis.

Rothchild Alissa C AC   Sissons James R JR   Shafiani Shahin S   Plaisier Christopher C   Min Deborah D   Mai Dat D   Gilchrist Mark M   Peschon Jacques J   Larson Ryan P RP   Bergthaler Andreas A   Baliga Nitin S NS   Urdahl Kevin B KB   Aderem Alan A  

Proceedings of the National Academy of Sciences of the United States of America 20160928 41


The regulation of host-pathogen interactions during Mycobacterium tuberculosis (Mtb) infection remains unresolved. MicroRNAs (miRNAs) are important regulators of the immune system, and so we used a systems biology approach to construct an miRNA regulatory network activated in macrophages during Mtb infection. Our network comprises 77 putative miRNAs that are associated with temporal gene expression signatures in macrophages early after Mtb infection. In this study, we demonstrate a dual role for  ...[more]

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