Genomics

Dataset Information

155

Function of deubiquitinase USP21 in mouse embryonic stem cell [ChIP-Seq]


ABSTRACT: Nanog is a master pluripotency factor of embryonic stem cells (ESCs). Stable expression of Nanog is required to maintain the stemness of ESCs, although Nanog is a short-lived protein and quickly degraded by the ubiquitin-dependent proteasome system (UPS). Here, we report that the deubiquitinase USP21 interacts with, deubiquitinates and stabilizes Nanog and therefore maintains the protein level of Nanog in mouse-ESCs (mESCs). Loss of USP21 results in Nanog destruction, mESCs differentiation and reduced the somatic cell reprogramming efficiency. USP21 is a transcriptional target of the LIF/STAT3 pathway and is downregulated upon differentiation. Moreover, differentiation cues promote ERK-mediated phosphorylation and dissociation of USP21 from Nanog, thus leading to Nanog degradation. Additionally, USP21 is recruited to gene promoters by Nanog to deubiquitinate histone H2A at K119 and thus facilitates Nanog-mediated gene expression. Together, our findings provide a regulatory mechanism by which extrinsic signals regulate mESC fate via deubiquitinating Nanog. Overall design: Examination of the function of USP21 in mouse embryonic stem cells E14 cell line.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Liu Zhenping  

PROVIDER: GSE79890 | GEO | 2016-10-05

SECONDARY ACCESSION(S): PRJNA317333

REPOSITORIES: GEO

altmetric image

Publications

The deubiquitinase USP21 maintains the stemness of mouse embryonic stem cells via stabilization of Nanog.

Jin Jiali J   Liu Jian J   Chen Cong C   Liu Zhenping Z   Jiang Cong C   Chu Hongshang H   Pan Weijuan W   Wang Xinbo X   Zhang Lingqiang L   Li Bin B   Jiang Cizhong C   Ge Xin X   Xie Xin X   Wang Ping P  

Nature communications 20161125


Nanog is a master pluripotency factor of embryonic stem cells (ESCs). Stable expression of Nanog is essential to maintain the stemness of ESCs. However, Nanog is a short-lived protein and quickly degraded by the ubiquitin-dependent proteasome system. Here we report that the deubiquitinase USP21 interacts with, deubiquitinates and stabilizes Nanog, and therefore maintains the protein level of Nanog in mouse ESCs (mESCs). Loss of USP21 results in Nanog degradation, mESCs differentiation and reduce  ...[more]

Similar Datasets

| GSE69987 | GEO
2012-04-05 | E-GEOD-34920 | ArrayExpress
2012-04-05 | E-GEOD-34918 | ArrayExpress
2012-04-05 | E-GEOD-34912 | ArrayExpress
| GSE34904 | GEO
2012-04-05 | E-GEOD-34904 | ArrayExpress
2012-07-31 | E-GEOD-39771 | ArrayExpress
2012-07-31 | E-GEOD-39770 | ArrayExpress
2012-04-11 | GSE36182 | GEO
2014-05-02 | E-GEOD-52899 | ArrayExpress