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MiRNA expression profiling in human glioma stem cell upon TUG1 inhibition

ABSTRACT: Glioblastomas show heterogeneous histological features. These distinct phenotypic states are thought to be associated with the presence of glioma stem cells (GSCs), which are highly tumorigenic and self-renewing sub-population of tumor cells that have different functional characteristics. We found that TUG1, a long non-coding RNA (lncRNA), plays pivotal roles in maintaining the self-renewal properties of GSC. Some lncRNAs are known to act as a miRNA sponge in the cytoplasm, where lncRNAs bind to miRNAs and quench their activity. To investigate miRNA expression profiling in GSC upon TUG1 inhibition, we have performed microarray experiment using SurePrint G3 Human miRNA 8x60K Microarray (G4872A, Agilent Technologies).

ORGANISM(S): Homo sapiens

PROVIDER: GSE79896 | GEO | 2016/11/23

SECONDARY ACCESSION(S): PRJNA317342

REPOSITORIES: GEO

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Genomic maps of TUG1 in human glioma stem cell

Project description:Glioblastomas show heterogeneous histological features. These distinct phenotypic states are thought to be originated by the glioma stem cells (GSCs), which are highly tumorigenic and self-renewing sub-population of tumor cells that have different functional characteristics. We found TUG1, a large non-coding RNA (lncRNA) is highly expressed in GSCs. TUG1 was reported to interact with PRC2 via its exon 2 and represses its target gene expression in trans. In order to identify the genetic loci enriched with TUG1 in GSC, we performed modified RNA pull-down assay coupled with promoter-microarray analysis using BrU-labeled TUG1.

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TGF-b-activated LncRNA LINC00115 is a critical regulator for glioma stem-like cell tumorigenicity

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Regulation of mitochondrial Ca2+ uniporter expression and calcium signalling by lncRNA Tug1 in cardiomyocytes

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2016-10-24 | E-GEOD-77493 | biostudies-arrayexpress

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2016-11-23 | GSE79897 | GEO

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