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Effect of milk intake on circulating plasma miRNAs of adult humans

ABSTRACT: This study examines circulating plasma miRNAs of adult human subjects before and after bovine milk intake. We sought to confirm and expand findings previously reported by Baier, et al., J Nutr, 2014, PMID 25122645, using the same samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE79960 | GEO | 2016/04/06

SECONDARY ACCESSION(S): PRJNA317497

REPOSITORIES: GEO

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Effect of milk intake on circulating plasma miRNAs of adult humans

Project description:This study examines circulating plasma miRNAs of adult human subjects before and after bovine milk intake. We sought to confirm and expand findings previously reported by Baier, et al., J Nutr, 2014, PMID 25122645, using the same samples. Plasma samples were provided by the Zempleni lab (see Baier, et al., J Nutr, 2014, PMID 25122645) and had been taken from five healthy donors prior to milk intake (T0) and at three hours post-intake (T3). miRNA profiles were generated for each sample by OpenArray (Thermo Fisher).

2016-04-06 | E-GEOD-79960 | biostudies-arrayexpress

Effect of milk intake on circulating PBMC miRNAs of adult humans

Project description:This study examines circulating PBMC miRNAs of adult human subjects before and after bovine milk intake. We sought to confirm and expand findings previously reported by Baier, et al., J Nutr, 2014, PMID 25122645, using the same samples. PBMC RNA samples were provided by the Zempleni lab (see Baier, et al., J Nutr, 2014, PMID 25122645) and had been taken from five healthy donors prior to milk intake (T0) and at six hours post-intake (T6). miRNA profiles were generated for each sample by OpenArray (Thermo Fisher).

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Effect of milk intake on circulating PBMC miRNAs of adult humans

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Genome wide DNA methylation profiling of post-exercise milk product intake during interval walking training.

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2017-06-09 | GSE76503 | GEO

Elevated dairy fat intake in lactating women alters milk lipid and fatty acids without detectible changes in expression of genes related to lipid uptake or synthesis

2015-02-24 | GSE66191 | GEO

Elevated dairy fat intake in lactating women alters milk lipid and fatty acids without detectible changes in expression of genes related to lipid uptake or synthesis

Project description:Previous work has demonstrated that elevated maternal lipid intake (particularly from dairy products) is associated with increased lipids and altered fatty acid profile in milk produced by healthy lactating women. We investigate our primary hypothesis that a maternal diet rich in full-fat dairy products would simultaneously increase milk lipid percent and expression of genes related to the uptake and/or de novo biosynthesis of milk lipids. Within a a randomized, crossover study we performed a microarray analysis comparing 6 lactating women with diets rich in full-fat or non-fat dairy products. Each woman consumed one of the two diets during timepoint one, and after a two week washout period, switched over to the other diet. The timepoint value indicates whether the particular dietary treatment was consumed first or second.

2015-02-24 | E-GEOD-66191 | biostudies-arrayexpress

RNA-Seq data from the milk somatic cell transcriptome of primiparus Assaf dairy sheep

Project description:This research investigates the influence of nutritional protein restriction (NPR) during prepuberty on FE and the milk transcriptome of dairy Assaf ewes during their first lactation. Additionally, it evaluates the differences in the milk transcriptome between lactating ewes with divergent FE using the feed conversion ratio (FCR) and residual feed intake (RFI) indices and assesses milk gene expression as a predictor of FE.

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Inadequate protein intake affects skeletal muscle transcript profiles in older humans

Project description:Inadequate dietary protein intake causes adverse changes in the morphology and function of skeletal muscle. These changes may be reflected in early alterations in muscle mRNA levels. Microarray analysis was used to assess whether inadequate protein intake differentially affects skeletal muscle transcript levels and expression profiles in older adults. Keywords: dietary intake response

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Analysis of gene expression changes in whole blood samples from young men after acute or short-term intake of probiotic yoghurt compared to acidified milk

Project description:Fermented dairy milks have been associated with many health benefits including the regulation of metabolic dysfunction. Different circulating clinical biomarkers have been used to explore the effect of fermented milks on metabolic health but the development of whole blood transcriptomics has recently been proposed as a source of novel biomarkers for this health outcome. In a randomised, cross-over study, we evaluate the changes in the whole blood transcriptome after the intake of a probiotic yoghurt compared to a milk acidified with gluconic acid in seven healthy young men. The effects of the dairy foods on whole blood gene expression were assessed at three time points during a 6 h postprandial test (800g single dose) and in the fasting state after a daily intake of the products over two-weeks (400g/d). RNA was extracted from Paxgene ® whole blood samples and sequenced on the Illumina HiSeq platform.

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Milk fat miRNome changes in response to LPS challenge in Holstein cows

Project description:Cows were fed a lactation diet at ad libitum intake (n = 6). At 27±3 days in milk, cows were injected with 50 µg of LPS E. coli in one healthy rear mammary quarter. Milk samples were collected just before LPS challenge (LPS-) and 6.5 h after LPS challenge (LPS+) from the same cows. Microarray analysis was performed using customized 8x60K ruminant miRNA microarrays to compare LPS- to LPS+ miRNome. MiRNome comparison between LPS- and LPS+ identified 37 differentially abundant miRNAs (q-value ≤ 0.05)

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