Transcriptomics,Genomics

Dataset Information

151

RNA-seq analyses of JMJD3c-MYOD1-induced myogenic cells and human skeletal myotubes


ABSTRACT: We generated the human ES lines, in which the genome-wide reduction of H3K27me3 can be induced by the ectopic expression of catalytic domain of histone demethylase JMJD3 with doxycycline treatment (JMJD3c-hESCs). The overexpression of JMJD3c enhances MYOD1-mediated myogenic differentiation of hESCs. We compared the gene expression patterns of the generated myogenic cells with those of human skeletal myotubes. Overall design: Doxycycline-treated JMJD3c-hESCs were differentiated into myogenic cells by MYOD1 overexpression. Human myoblast HSMM cells were differentiated into myotubes with 2% horse serum.

INSTRUMENT(S): Illumina MiSeq (Homo sapiens)

SUBMITTER: Tomohiko Akiyama 

PROVIDER: GSE80106 | GEO | 2016-10-18

SECONDARY ACCESSION(S): PRJNA317930

REPOSITORIES: GEO

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Publications

Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells.

Akiyama Tomohiko T   Wakabayashi Shunichi S   Soma Atsumi A   Sato Saeko S   Nakatake Yuhki Y   Oda Mayumi M   Murakami Miyako M   Sakota Miki M   Chikazawa-Nohtomi Nana N   Ko Shigeru B H SB   Ko Minoru S H MS  

Development (Cambridge, England) 20161001 20


Harnessing epigenetic regulation is crucial for the efficient and proper differentiation of pluripotent stem cells (PSCs) into desired cell types. Histone H3 lysine 27 trimethylation (H3K27me3) functions as a barrier against cell differentiation through the suppression of developmental gene expression in PSCs. Here, we have generated human PSC (hPSC) lines in which genome-wide reduction of H3K27me3 can be induced by ectopic expression of the catalytic domain of the histone demethylase JMJD3 (cal  ...[more]

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