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RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected EVT cells

ABSTRACT: We used high throughput sequencing to analyze the transcriptional profiling of EVT. By comparing the transcriptional profiling of EVT with or without H19 knockdown, numerous genes showed significantly altered expression as a result of H19 repression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE80237 | GEO | 2017/02/14

SECONDARY ACCESSION(S): PRJNA318378

REPOSITORIES: GEO

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RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected myotubes

2015-11-09 | GSE72941 | GEO

RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected myotubes

Project description:We used high throughput sequencing to analyze the transcriptional profiling of myotubes. By comparing the transcriptional profiling of myotubes with or without H19 knockdown,1131 genes or transcripts were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 508 genes or transcripts were found at least 1.5-fold down-regulated in siH19 over siCon cells. 40h following initiation of differentiation, myotubes were transfected with either control siRNA or siH19. 48h later after transfection, total RNA was extracted for library preparation and RNA-seq analysis to compare trancript profiles between siCon and siH19 cells.

2015-11-09 | E-GEOD-72941 | biostudies-arrayexpress

RNA-seq analysis reveals profound changes in transcript profiles between siCon and siH19 transfected aortic smooth muscle cells (AoSMC)

Project description:We used high throughput sequencing to analyze the gene expression profiling of human aortic smooth muscle cells (AoSMC). By comparing the gene expression profiling of AoSMC with or without H19 knockdown, 2940 genes were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 2790 genes were found at least 1.5-fold down-regulated in siH19 over siCon cells.

2021-02-13 | GSE110556 | GEO

RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected uterine smooth muscle cells (USMC)

Project description:We used high throughput sequencing to analyze the gene expression profiling of human uterine smooth muscle cells (USMC). By comparing the gene expression profiling of USMC with or without H19 knockdown, 215 genes were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 228 genes were found at least 1.5-fold down-regulated in siH19 over siCon cells.

2019-10-01 | GSE110557 | GEO

RNA-seq analysis reveals profound changes in transcript profiles between siCon and siH19 transfected aortic smooth muscle cells (AoSMC)

Project description:RNA-seq analysis reveals profound changes in transcript profiles between siCon and siH19 transfected aortic smooth muscle cells (AoSMC)

| PRJNA433982 | ENA

RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected uterine smooth muscle cells (USMC)

Project description:RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected uterine smooth muscle cells (USMC)

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Sharkey trophoblast

Project description:Transcriptional profiling comparison of human primary villous trophoblast and extra-villous trophoblast (VT and EVT respectively) cells, isolated from the placenta at 8 to 12 weeks gestation, with complimentary transcriptional profiling of choriocarcinoma cell lines JEG-3 and JAR. Based on phenotypic markers, JEG is frequently used as a model for EVT and JAR is used as a model for VT.

2011-04-28 | E-MTAB-429 | biostudies-arrayexpress

Transcriptomic Drivers of Differentiation, Maturation, and Polyploidy in Human Extravillous Trophoblast [SNP array]

Project description:During pregnancy, normal extravillous trophoblast (EVT) invade the endomyometrium of the maternal uterus and remodel the spiral arteries to control blood supply and nutrient transfer. Recent reports have described first trimester EVT as polyploid and senescent. Here we extend these earlier reports by performing comprehensive profiling of the genomic organization and transcriptome of first trimester EVT and term EVT. We define pathways and gene regulatory networks involved in both initial differentiation and maturation of this important trophoblast lineage at the maternal-fetal interface. Our results suggest that like first trimester EVT, term EVT undergo senescence and endoreduplication, are primarily tetraploid, and lack high rates of copy number variations. Additionally, we have highlighted senescence and polyploidy-related genes, pathways, networks, and transcription factors that appeared to be important in normal EVT differentiation and maturation and have validated a critical role for the unfolded protein response in EVT differentiation.

2021-08-11 | GSE173369 | GEO

Transcriptomic Drivers of Differentiation, Maturation, and Polyploidy in Human Extravillous Trophoblast [RNA-seq]

2021-08-11 | GSE173323 | GEO

RNA Seq analyzed the transcriptional difference between the ICSI and ICAHCI embryo in blastocyst and placenta

Project description:Our lab first derived mouse androgenetic haploid embryonic stem cells (AG-haESCs) and demonstrated that AG-haESCs can be used as an “artificial spermatids” to generate gene-edited semi-cloned (SC) mice through intracytoplasmic injection (ICAHCI) into mature oocyte, even though the birth efficiency is very low. Further we proved that H19-DMR and IG-DMR were the main barrier to generate viable mice through androgenetic and parthenogenetic haESCs. More importantly, AG-haESCs mediated SC technology combined with CRISPR-Cas9 is a powerful tool to generate gene-modified mouse models and carry out genetic screening at organismal level. However, it is still not clear how the H19-DMR and IG-DMR coordinately regulate SC embryo development. Here, we found that the H19-DMR and IG-DMR regulate the development of SC embryos in spatio-temporal scales. Firstly, we found that the H19-DMR and IG-DMR are not indispensable for the development of preimplantation of SC embryos. Secondly, H19-DMR is essential for the development of SC embryos in mid-gestation and IG-DMR takes effect in late-gestation. Further, the maintenance of paternal H19-DMR methylation status and deletion of paternal H19 transcription unit play a key role in the structures and transport functions of SC embryo placenta. Importantly, AG-haESCs carrying triple deletions, including H19, H19-DMR and IG-DMR, can further improve the efficiency in generation of viable, normal-size, and fertile mice.

2019-09-17 | GSE132254 | GEO

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