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Comparison of transcriptomes of mouse cumulus cells collected from mice before and after 6-h of hCG priming.

ABSTRACT: We sought to identify genes that are regulated by the ovulatory signals in mouse cumulus cells. We compared the transcriptomes between cumulus cells colected from mice before (hCG-0h) and after 6 h (hCG-6h) of hCG injection.

ORGANISM(S): Mus musculus

PROVIDER: GSE80255 | GEO | 2016/08/01

SECONDARY ACCESSION(S): PRJNA318400

REPOSITORIES: GEO

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Comparison of transcriptomes of mouse cumulus cells collected from mice before and after 6-h of hCG priming.

Project description:We sought to identify genes that are regulated by the ovulatory signals in mouse cumulus cells. We compared the transcriptomes between cumulus cells colected from mice before (hCG-0h) and after 6 h (hCG-6h) of hCG injection. 3 individual cumulus cell samples of hCG-0h and hCG-6h were collected and subjected to microarray study. Copmarison was done between hCG-0h and hCG-6h groups.

2016-08-01 | E-GEOD-80255 | biostudies-arrayexpress

Rhesus monkey cumulus cells revert to a mural granulosa cell state following an ovulatory stimulus

Project description:Follicular somatic cells (mural granulosa cells and cumulus cells) and the oocyte communicate through paracrine interactions and through direct gap junctions between oocyte and cumulus cells. Considering that mural and cumulus cells arise through a common developmental pathway and that their differentiation is essential to reproductive success, understanding how these cells differ is a key aspect to understanding their critical functions. Changes in global gene expression before and after an ovulatory stimulus were compared between cumulus and mural granulosa cells to test the hypothesis that mural and cumulus cells are highly differentiated at the time of an ovulatory stimulus and further differentiate during the periovulatory interval. The transcriptomes of the two cell types were markedly different (>1500 genes) before an ovulatory hCG bolus but converged after ovulation to become completely overlapping. The predominant transition was for the cumulus cells to become more like mural cells after hCG. This indicates that the differentiated phenotype of the cumulus cell is not stable and irreversibly established but may rather be an ongoing physiological response to the oocyte.

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Rhesus monkey cumulus cells revert to a mural granulosa cell state following an ovulatory stimulus

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Cumulus-oocyte complex temporal expression

Project description:Cumulus-oocyte complexes were isolated a seperate time-points to generate temporal complexes. Targets from two biological replicates at each time point (0h, 8h, 16h post-hCG treatment) were generated and the expression profiles were determined using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. Comparisons between the sample groups allow the identification of genes with temporal expression patterns. Keywords: time course

2006-02-17 | GSE4260 | GEO

Transcription profiling of cumulus cell oocyte complexes isolated at different time points of ovulation to identify differentially expressed genes.

Project description:Cumulus-oocyte complexes were isolated at seperate time-points to generate temporal complexes. Targets from two biological replicates at each time point (0h, 8h, 16h post-hCG treatment) were generated and the expression profiles were determined using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. Comparisons between the sample groups allow the identification of genes with temporal expression patterns. Experiment Overall Design: 2 eCG-primed (48h) pooled cumulus-oocyte complexes, 2 eCG-primed (48h) hCG-treated (8h) pooled cumulus-oocyte complexes, and 2 eCG-primed (48h) hCG-treated (16h) pooled cumulus-oocyte complexes replicates were analyzed

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Transcriptome analysis of bovine cumulus cells during first 6 hrs of in vitro maturation

Project description:Study the effect of in vitro maturation system on the transcriptomics of bovine cumulus cells during incubation of first 6 hrs. 3 biological replicates of cumulus cells derived from cumulus oocyte complexes (COCs) after 6 hrs of in vitro matutaion(Ct-6H) vs. cumulus cells before maturation (Ct-0H, REFERENCE) were contrasted. Each replicate was subjected to a dye-swap procedure.

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Genome-wide maps of H3K27Ac occupancy on chromatin in mouse ovaries before and after hCG treatment.

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Comparing the transcriptome in cumulus and granulosa cells

Project description:Comparing cumulus and granulosa cells from human individual pre-ovulatory follicles after controlled ovarian stimulation

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GnHR agonist trigger versus hCG trigger in final ovulation induction

Project description:The objective of this experiment was to explore differences in follicle transcriptomes in patients having oocyte maturation induced with either a bolus of hCG or a GnRH agonist. Upon oocyte retrieval, mural granuloasa cells and cumulus cells were isolated, allowing for comparison of the follicular transcriptomes between patients treated with eihter hCG or the GnHR agonist.

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Transcriptomic analysis of corona radiata cells after hormonal stimulation (PMSG/hCG)

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