Genomics

Dataset Information

158

LaminB1 DamID in undifferentiated mouse C2C12 myoblasts (ATCC, Lot 59501261) and differentiated C2C12 myotubes


ABSTRACT: Maps of genomic regions in proximity to the nuclear lamina were determined in undifferentiated C2C12 myoblasts (MBs) and 6 day differentiated C2C12 myotubes (MTs) using DamID with a Dam-Lamin B1-encoding lentivirus. Overall design: Examination of Lamin B1-chromatin contacts in one cell line in undifferentiated myoblast and differentiated myotube conditions.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Jose Ignacio de las Heras  

PROVIDER: GSE80328 | GEO | 2016-06-17

SECONDARY ACCESSION(S): PRJNA318617

REPOSITORIES: GEO

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Publications

Tissue-Specific Gene Repositioning by Muscle Nuclear Membrane Proteins Enhances Repression of Critical Developmental Genes during Myogenesis.

Robson Michael I MI   de Las Heras Jose I JI   Czapiewski Rafal R   Lê Thành Phú P   Booth Daniel G DG   Kelly David A DA   Webb Shaun S   Kerr Alastair R W ARW   Schirmer Eric C EC  

Molecular cell 20160602 6


Whether gene repositioning to the nuclear periphery during differentiation adds another layer of regulation to gene expression remains controversial. Here, we resolve this by manipulating gene positions through targeting the nuclear envelope transmembrane proteins (NETs) that direct their normal repositioning during myogenesis. Combining transcriptomics with high-resolution DamID mapping of nuclear envelope-genome contacts, we show that three muscle-specific NETs, NET39, Tmem38A, and WFS1, direc  ...[more]

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