Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis of human induced pluripotent stem cell-derived (hiPSC) cardiomyocytes in 2D versus 3D (Engineered heart tissue) format

ABSTRACT: Analysis of cardiomyocytes cultivated in 2D or age-matched 3D (Engineered heart tissue, EHT) format.

ORGANISM(S): Homo sapiens

PROVIDER: GSE80390 | GEO | 2016/04/20

SECONDARY ACCESSION(S): PRJNA318920

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Contractile work contributes to maturation of energy metabolism in hiPSC-derived cardiomyocytes

Project description:Energy metabolism is a key aspect of cardiomyocyte biology. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are a promising tool for biomedical application, but they are immature and have not undergone metabolic shift related to early postnatal development. Cultivation of hiPSC-CM in 3D engineered heart tissue (EHT) format leads to morphological maturation. This study compared the mitochondrial and metabolic state of hiPSC-CM in standard 2D culture and the EHT format and determined the influence of contractile activity. HiPSC-CM in EHTs showed ~2-fold higher number of mitochondria (electron microscopy), mitochondrial mass (mitotracker), DNA (Mt-ND1, Mt-ND2), and protein abundance (proteome) than in 2D culture. While hiPSC-CM exhibited the principal ability to use glucose, lactate and fatty acids as energy substrates irrespective of culture format, hiPSC-CM in 3D performed more oxidation of glucose, lactate and fatty acid, and less anaerobic glycolysis. The increase in mitochondrial mass and DNA in 3D was diminished by pharmacological inhibition of contractile force, suggesting that contractile work participates in mitochondrial development hiPSC-CM. In conclusion, contractile work in the EHT format contributes to metabolic maturation of hiPSC-CM.

2018-03-06 | PXD008205 | Pride

Transcriptome Analysis of Non-Human Primate Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 2D Monolayer Culture versus 3D Engineered Heart Tissue

Project description:In this study, we utilized both monolayer culture (2D) and engineered heart tissue (EHT) (3D) with NHP iPSC-CMs, which served as surrogates of direct cell injection and cell patch in vivo during exposure to hypoxic conditions, and the outcomes were compared at the transcriptome level. We found the 3D EHT model was more sensitive to ischemic conditions and similar to the native in vivo myocardium in terms of cell-extracellular matrix/cell-cell interactions, energy metabolism, and paracrine signaling.

2021-05-11 | GSE156237 | GEO

Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures.

Project description: Not available

| S-EPMC7246991 | biostudies-literature

Blinded Contractility Analysis in hiPSC-Cardiomyocytes in Engineered Heart Tissue Format: Comparison With Human Atrial Trabeculae.

Project description: Not available

| S-EPMC5837217 | biostudies-literature

3D Laparoscopy Versus 2D Laparoscopy

Project description:* To compare surgical and oncological outcomes in patients underwent to colorectal resection with 3D vs 2D laparoscopic technique. * To evaluate the visual overload in surgeons using 3D laparoscopic technique.

| 2220465 | ecrin-mdr-crc

Clinical randomized study of 3D and 2D laparoscopic radical resection for colorectal cancer.

Project description:Interventions: 2D group:2D laparoscopic surgery;3D group:3D laparoscopic surgery Primary outcome(s): operation time;The amount of bleeding;Number of lymph nodes;Postoperative gastrointestinal function recovery time;Postoperative complications Study Design: Randomized parallel controlled trial

| 2663755 | ecrin-mdr-crc

Expression data from 2D and 3D HepG2 cells

Project description:Expression data from HepG2 cultured in 2D monolayer cultures and 3D Matrigel cultures We performed this study to understand differences in gene expression profiles of 2D and 3D HepG2 cultures

2019-08-31 | GSE128763 | GEO

Protein Profiling of human iPSC-derived cardiomyocytes with mutations in ACTN2

Project description:Genetic variants in α-actinin 2 (ACTN2) are associated with several forms of (cardio)myopathy. Here, we analyzed the proteome of 2D and EHT cultured cardiomyocytes derived from mutant-expressing (ACTN2mut) and a wild-type-expressing (ACTN2wt) hiPSC lines, both created with CRISPR/Cas9 genetic tools.

2022-10-13 | PXD034258 | Pride