Project description:The adverse effect of CSDS on the DG neurogenesis is expected to affect the properties of the resident NSCs/NPCs.We used neurosphere culture system, a well-established ex vivo model to study neurogenesis. We collected proliferating neurospheres and differentiating NSCs/NPCs after 7 days of plating and isolated RNA for microRNA array to identify the altered miRNAs

2019-07-17 | GSE132822 | GEO

Gene Expression data from the differentiating and proliferating neural stem/progenitor cells

Project description:The adverse effect of CSDS on the DG neurogenesis is expected to affect the properties of the resident NSCs/NPCs.We used neurosphere culture system, a well-established ex vivo model to study neurogenesis. We collected proliferating neurospheres and differentiating NSCs/NPCs from two stages, i.e. early (after 2 days) and late (after 7 days) and isolated RNA for gene arrays.

2019-07-17 | GSE132820 | GEO

Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.

Project description: Not available

| S-EPMC7252506 | biostudies-literature

Mediator complex interaction partners organize the transcriptional network that defines neural stem cells

Project description:Mediator complex regulates transcription by connecting enhancers to promoters. High Mediator binding density defines super enhancers, which regulate cell-identity genes and oncogenes. Protein interactions of Mediator may explain its role in these processes but have not been identified comprehensively. Here we purify Mediator from neural stem cells (NSCs) and identify 75 protein-protein interaction partners. We identify super enhancers in NSCs and show that Mediator-interacting chromatin modifiers colocalise with Mediator at enhancers and super enhancers. Transcription factor families with high affinity for Mediator dominate enhancers and super enhancers and can explain genome-wide Mediator localization. We identify E-box transcription factor Tcf4 as a key regulator of NSCs. Tcf4 interacts with Mediator, colocalises with Mediator at super enhancers and regulates neurogenic transcription factor genes with super enhancers and broad H3K4me3 domains. Our data suggest that high binding-affinity for Mediator is an important organizing feature in the transcriptional network that determines NSC identity.

2019-05-06 | PXD013546 | Pride

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