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Knock-down of the methyltransferase Kmt6 reliefs H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Fusarium fujikuroi

PROVIDER: GSE80479 | GEO | 2016/07/04

SECONDARY ACCESSION(S): PRJNA319028

REPOSITORIES: GEO

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Knock-down of the methyltransferase Kmt6 relieves H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi.

Project description: Not available

| S-EPMC5118082 | biostudies-literature

Knock-down of the methyltransferase Kmt6 reliefs H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-07-04 | E-GEOD-80479 | biostudies-arrayexpress

Knock-down of the methyltransferase Kmt6 reliefs H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi [ChIP-seq]

Project description:We performed ChIP-seq of H3K27me3 in wild type Fusarium fujikuroi grown in synthetic ICI medium with low nitrogen conditions. Three replicates of F. fujikuroi wild-type strain were grown in low nitrogen. ChIP-Seq was performed with anti-H3K27me3 antibody.

2016-07-04 | E-GEOD-80478 | biostudies-arrayexpress

Knock-down of the methyltransferase Kmt6 reliefs H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi [ChIP-seq]

Project description:We performed ChIP-seq of H3K27me3 in wild type Fusarium fujikuroi grown in synthetic ICI medium with low nitrogen conditions.

2016-07-04 | GSE80478 | GEO

Knock-down of the methyltransferase Kmt6 reliefs H3K27me3 and induces expression of cryptic otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi [gene expression]

Project description:Filamentous fungi produce a vast array of secondary metabolites (SMs) and some of them are applied in agriculture or pharmacology. Recent sequencing of the rice pathogen Fusarium fujikuroi revealed the presence of far more SM-encoding genes than known products. SM production is energy-consuming and thus tightly regulated, leaving the majority of SM gene clusters silent under laboratory conditions. It is now well established that one important regulatory layer in SM biosynthesis involves histone modifications that render the genes either silent or poised for transcription. In this study, we show that the majority of the putative SM gene clusters in F. fujikuroi are located within facultative heterochromatin marked by H3K27me3. In this study, we performed comparative transcriptomics of a knock-down mutant of the responsible methyltransferase Kmt6 involved in H3K27 methylation grown on either solid complete medium or solid synthetic ICI medium. Overall four so far cryptic and otherwise silent putative SM gene clusters were significantly induced in the KMT6kd strain accompanied by reduced H3K27me3 levels at the respective gene loci and accumulation of novel metabolites. One of the four putative SM gene clusters, the STC5 gene cluster, was analysed in detail and heterologous expression of the key enzyme allowed for the identification of the first pathway-specific intermediate (1R,4R,5S)-guaia-6,10(14)-diene. 2 strains were analysed in overall two conditions, and each with 3 biological replicates

2016-07-04 | E-GEOD-80477 | biostudies-arrayexpress

Knock-down of the methyltransferase Kmt6 reliefs H3K27me3 and induces expression of cryptic otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi [gene expression]

2016-07-04 | GSE80477 | GEO

Knock-down of the methyltransferase Kmt6 reliefs H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi

Project description:Knock-down of the methyltransferase Kmt6 reliefs H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi

| PRJNA319028 | ENA

The Fusarium graminearum histone H3 K27 methyltransferase KMT6 regulates development and expression of secondary metabolite gene clusters.

Project description: Not available

| S-EPMC3814326 | biostudies-literature

The Fusarium graminearum Histone H3 K27 Methyltransferase KMT6 Regulates Development and Expression of Secondary Metabolite Gene Clusters

Project description:We performed ChIP-seq of histone modifications and RNA-seq in WT and kmt6 mutant Fusarium graminearum grown in high and low nitrogen conditions. Two replicates of RNA seq for WT and kmt6 strains at high and low nitrogen. Four histone modification ChIPs in WT and kmt6 mutant strains at high and low nitrogen, plus two histone modifications in a kmt6+ complemented strain at high nitrogen.

2014-02-27 | E-GEOD-50689 | biostudies-arrayexpress

The Fusarium graminearum Histone H3 K27 Methyltransferase KMT6 Regulates Development and Expression of Secondary Metabolite Gene Clusters

Project description:We performed ChIP-seq of histone modifications and RNA-seq in WT and kmt6 mutant Fusarium graminearum grown in high and low nitrogen conditions.

2014-02-27 | GSE50689 | GEO

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