Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PRC2 preserves cellular plasticity and restricts intestinal secretory lineage commitment (RNA-seq)

ABSTRACT: These data include RNA Seq data generated from wild type and Eed Ko intestinal crypts from AhCre and AhCreEedf/f mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE80503 | GEO | 2017/01/16

SECONDARY ACCESSION(S): PRJNA319243

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

PRC2 preserves cellular plasticity and restricts intestinal secretory lineage commitment

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-01-16 | GSE80539 | GEO

PRC2 preserves intestinal progenitors and restricts secretory lineage commitment.

Project description: Not available

| S-EPMC5090213 | biostudies-literature

PRC2 preserves cellular plasticity and restricts intestinal secretory lineage commitment (ChIP-seq)

Project description:These data include the genome wide occupancy of histone modifications and target protein by ChIP sequencing in mouse crypt cells.

2017-01-16 | GSE80502 | GEO

PRC2 preserves cellular plasticity and restricts intestinal secretory lineage commitment

Project description:PRC2 preserves cellular plasticity and restricts intestinal secretory lineage commitment

| PRJNA319238 | ENA

Gene expression profiling of Eed knockout and Ezh2 knockout small intestinal crypts [RNA-seq]

Project description:Polycomb-mediated gene repression plays an important role in adult stem cell maintenance. We knocked out (using the inducible AhCre-LoxP system) Polycomb genes Eed and Ezh2 in the intestine for 6 weeks, after which crypts - the small intestinal stem cell zone - were harvested and RNA sequenced. We found Wnt, Notch and cell cycle pathways to be affected in Eed knockout (KO) but not Ezh2 KO crypts. Direct targets of Eed were determined by comparing this data with ChIP-sequencing. Small intestinal crypt mRNA profiles of 6 weeks-induced 12 weeks old Eed KO, Ezh2 KO and WT mice (all triplicates) as well as 10 days-induced Eed KO and WT organoids (duplicates) were generated by RNA sequencing over two runs and using IlluminaHiseq2000 and Hiseq2500.

2016-07-01 | E-GEOD-81576 | biostudies-arrayexpress

Gene expression profiling of Eed knockout and Ezh2 knockout small intestinal crypts [RNA-seq]

2016-07-01 | GSE81576 | GEO

Gene expression profiling of Eed knockout and Ezh2 knockout small intestinal crypts [ChIP-seq]

Project description:Polycomb-mediated gene repression plays an important role in adult stem cell maintenance. Direct targets of the Polycomb repressive complex PRC2 in th intestinal epithelium were revealed by performing ChIP-sequencing on crypt samples isolated from wild type murine small intestines. The resulting list of H3K27me3-enriched genes were compared with RNA-sequencing data from wild type and Eed knockout crypts. Crypts were isolated from wild type murine intestinal epithelium and subjected to ChIP using anti-H3K27me3 and anti-H3K27Ac antibodies, after which DNA isolated from extracted immunocomplexes was sequenced.

2016-07-01 | E-GEOD-81577 | biostudies-arrayexpress

Gene expression profiling of Eed knockout and Ezh2 knockout small intestinal crypts [ChIP-seq]

2016-07-01 | GSE81577 | GEO

Daxx maintains endogenous retroviral silencing and restricts cellular plasticity in vivo.

Project description: Not available

| S-EPMC7406367 | biostudies-literature

PRC1 preserves epidermal tissue integrity independently of PRC2.

Project description: Not available

| S-EPMC6317312 | biostudies-literature