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Identification of an NKX3.1-G9a-UTY regulatory network that controls prostate differentiation (Mouse_15M_SV_Affy)

ABSTRACT: Analysis of transcriptome of seminal vesicle from 15-month-old Nkx3.1+/+ mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE80600 | GEO | 2016/06/24

SECONDARY ACCESSION(S): PRJNA321545

REPOSITORIES: GEO

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Identification of an NKX3.1-G9a-UTY regulatory network that controls prostate differentiation

Project description:To investigate the role of NKX3.1 in prostate differentiation, we employed transcriptome analysis of mouse seminal vesicle (from 15-month-old Nkx3.1+/+ mice); mouse prostate (from 4-month-old Nkx3.1+/+ and Nkx3.1-/- mice); human prostate cells (RWPE1 cells engineered with empty vector (altered pTRIPZ), NKX3.1 wild type over-expression, and NKX3.1 (T164A) mutant over-expression); and tissue recombinants (generated from combining engineered mouse epithelial cells (seminal vesicle epithelial cells or prostate epithelial cells from 2-month-old mice) and rat UGS mesenchymal cells). Mouse tissue or human cells were snap frozen for subsequent molecular analysis. This SuperSeries is composed of the SubSeries listed below.

2016-06-24 | GSE81440 | GEO

Identification of an NKX3.1-G9a-UTY regulatory network that controls prostate differentiation

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Identification of an NKX3.1-G9a-UTY regulatory network that controls prostate differentiation (Mouse_15M_SV_Affy)

Project description:Analysis of transcriptome of seminal vesicle from 15-month-old Nkx3.1+/+ mice. Total RNA obtained from seminal vesicle from 15-month-old Nkx3.1+/+ mice. Seminal vesicle was harvested, RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) and purified using an RNeasy kit (Qiagen, Chatsworth, CA). cDNA was labeled using a BioArray High-Yield RNA transcript labeling kit (Enzo Life Sciences, Farmingdale, NY) and hybridized to Affymetrix GeneChips (Mu74AV2).

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Identification of an NKX3.1-G9a-UTY regulatory network that controls prostate differentiation (Mouse_4M-Prostate_RNA-Seq)

Project description:Analysis of transcriptome of prostate tissue from 4-month-old Nkx3.1 +/+ and Nkx3.1 -/- mice.

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Identification of an NKX3.1-G9a-UTY regulatory network that controls prostate differentiation (Mouse_4M-Prostate_RNA-Seq)

Project description:Analysis of transcriptome of prostate tissue from 4-month-old Nkx3.1 +/+ and Nkx3.1 -/- mice. Total RNA obtained from prostate tissues from 4-month-old Nkx3.1 +/+ and Nkx3.1 -/- mice. Prostate tissues were harvested and processed for RNA isolation and transcriptome analysis using the MagMAX RNA isolation kit (Ambion).

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Identification of an NKX3.1-G9a-UTY regulatory network that controls prostate differentiation (Mouse_Recomb_RNA-Seq)

Project description:Analysis of transcriptome of tissue recombinants (mouse seminal vesicle epithelial [SVE] cells or prostate epithelial [PE] cells, and rat urogenital sinus [UGS] mesenchymal cells) grown under the kidney capsule in athymic nude mice for 3 months. Total RNA obtained from tissue recombinants generated from combining engineered mouse epithelial cells (SVE or PE from 2-month-old C57Bl/6J mice) and rat UGS mesenchymal cells. Tissue recombinants were harvested and processed for RNA isolation and transcriptome analysis using the RNeasy kit (Qiagen).

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Identification of an NKX3.1-G9a-UTY regulatory network that controls prostate differentiation (Human_RWPE1_RNA-Seq)

Project description:Analysis of transcriptome of human RWPE1 cells over-expressing wild type NKX3.1 and mutant NKX3.1 (T164A).

2016-06-24 | GSE81439 | GEO

Identification of an NKX3.1-G9a-UTY regulatory network that controls prostate differentiation (Human_RWPE1_RNA-Seq)

Project description:Analysis of transcriptome of human RWPE1 cells over-expressing wild type NKX3.1 and mutant NKX3.1 (T164A). Total RNA obtained from RWPE1 cells engineered with empty vector (altered pTRIPZ), NKX3.1 wild type over-expression, and NKX3.1 (T164A) mutant over-expression. Engineered RWPE1 cells were harvested and processed for RNA isolation and transcriptome analysis using the MagMAX RNA isolation kit (Ambion).

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