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Changes in relative transcript amounts caused by ∆phoU2::kanrpsL+ and double ∆phoU2::kanrpsL+ ∆phoU1::Pcerm mutations in Streptococcus pneumoniae

ABSTRACT: Unlike most bacteria, Streptococcus pneumoniae (pneumococcus) has two evolutionarily distinct ABC transporters (Pst1 and Pst2) for inorganic phosphate (Pi) uptake. The genes encoding a two-component regulator (PnpRS) are located immediately upstream of the pst1 operon. Both the pst1 and pst2 operons encode putative PhoU regulators (PhoU1 and PhoU2) at their ends.his study addresses why S. pneumoniae contains dual Pi uptake systems and the regulation and contribution of the Pst1 and Pst2 systems in conditions of high (mM) Pi amount and low (μM) Pi amount. To determine whether PhoU1 or PhoU2 regulates pnpRS, pst1, or pst2 operons. we performed RNA-Seq analyses of ΔphoU2::kanrpsL+ sinlge mutant and ΔphoU2::kanrpsL+ ΔphoU1::Pcerm double mutant compared to the isogenic encapsulated strain cep::kanrpsL+. Unexpectedly, only ΔphoU2::kanrpsL+ indulces the pst1 operon, but not pst2 or pnpRS operon. Addtional PhoU1 deletion has no effect on pst1, pst2, or pnpRS operons.

ORGANISM(S): Streptococcus pneumoniae

PROVIDER: GSE80637 | GEO | 2017/02/14

SECONDARY ACCESSION(S): PRJNA319551

REPOSITORIES: GEO

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S1-Domain RNA Binding Protein (CvfD) Is a New Post-Transcriptional Regulator That Mediates Cold Shock, Phosphate Transport, and Virulence in Streptococcus pneumoniae D39

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S1-Domain RNA Binding Protein (CvfD) Is a New Post-Transcriptional Regulator That Mediates Cold Shock, Phosphate Transport, and Virulence in Streptococcus pneumoniae D39

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2015-04-23 | E-GEOD-65673 | biostudies-arrayexpress

Fucose-mediated transcriptional activation of the fcs operon by FcsR in Streptococcus pneumoniae

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Project description:Abstract: Transcriptome comparison of the Streptococcus pneumoniae strain D39 grown in the presence of either lactose or galactose with that of the strain grown in the presence of glucose, revealed the elevated expression of various genes and operons, including the lac gene cluster that is organized into two operons i.e. lac operon-I (lacABCD) and lac operon-II (lacTFEG). Deletion of the DeoR family transcriptional regulator lacR that is present downstream of the lac gene cluster, revealed elevated expression of lac operon-I, even in the absence of lactose. This suggests a function of LacR as a transcriptional repressor of lac operon-I that encodes enzymes involved in the Tagatose-6-P pathway in the absence of lactose or galactose. Deletion of lacR did not affect the expression of lac operon-II that encodes for a lactose-specific PTS. This finding was further confirmed by ?-galactosidase assays with PlacA-lacZ and PlacT-lacZ in the presence of either lactose or glucose as a sole carbon source in the medium. This suggests the presence of another transcriptional regulator in the regulation of lac operon-II, which could be the BglG-family transcriptional antiterminator LacT. We demonstrate the role of LacT as a transcriptional activator of lac operon-II in the presence of lactose and CcpA-independent regulation of the lac gene cluster in S. pneumoniae. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-06-20 | E-GEOD-58184 | biostudies-arrayexpress

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2014-06-20 | GSE58184 | GEO

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2019-07-01 | GSE122971 | GEO

A conserved two-component signal transduction system controls the response to phosphate starvation in Bifidobacterium breve UCC2003

Project description:This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by phoRP in Bifidobacterium breve UCC2003, which controls the response to phosphate (Pi) starvation. The response regulator PhoP was shown to bind to the promoter region of pstSCAB, specifying a predicted Pi transporter system, as well as that of phoU, which specifies a putative Pi-responsive regulatory protein. This interaction is assumed to cause transcriptional activation under conditions of Pi limitation. The phoRP genes appear to be subject to positive auto-regulation, and with pstSCAB and phoU, represent the complete regulon controlled by the phoRP-encoded 2CRS in B. breve UCC2003. Determination of the minimal PhoP binding region combined with bioinformatic analysis revealed the probable recognition sequence of PhoP, designated here as the PHO box, which together with phoRP is conserved among many high GC-content Gram+ bacteria. The importance of the phoRP 2CRS in the response of B. breve to Pi starvation conditions was confirmed by generating a B. breve::phoP insertion mutant which exhibited decreased growth under phosphate-limiting conditions as compared to its parent strain UCC2003.

2012-09-06 | GSE34983 | GEO

Comparison of the Streptococcus pneumoniae D39 grown in AM17 compared to D39 wild-type grown in M17

Project description:In this study, we have explored the impact of ascorbic acid on the transcriptome of Streptococcus pneumoniae D39. The expression of several genes and operons, including the ula operon (which has been previously shown to be involved in ascorbic acid utilization), the AdcR regulon (which has been previously shown to be involved in zinc transport and virulence) and a PTS operon (which we denote here as ula2 operon) were altered in the presence of ascorbic acid. The ula2 operon consists of five genes, including the transcriptional activator ulaR2. Our M-NM-2-galactosidase assay data and transcriptome comparison of the ulaR2 mutant with the wild-type demonstrated that the transcriptional activator UlaR2 in the presence of ascorbic acid activates the expression of the ula2 operon. We further predict a 16-bp regulatory site (5M-bM-^@M-^Y-ATATTGTGCTCAAATA-3M-bM-^@M-^Y) for UlaR2 binding in the Pula2. Furthermore, we have explored the effect of ascorbic acid on the expression of the AdcR regulon. Our ICP-MS analysis showed that addition of ascorbic acid to the medium causes zinc starvation in the cell that leads to the activation of the AdcR regulon. Comparison of the Streptococcus pneumoniae D39 compared to D39 wild type in M17 medium+ 10mM ascorbic acid (AM17) Two conditions design comparision of one strain including a dye swap

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Comparison of the Streptococcus pneumoniae D39 ulaR2 Mutant compared to D39 wild-type

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2015-01-21 | E-GEOD-64106 | biostudies-arrayexpress

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