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Gene expression for treatment of bioceramic water

ABSTRACT: Increased oxidative stress induces impairment of the antioxidant defense systems and defects in regeneration of injured tissues. We aim to evaluate the effects of antioxidant bioactive ceramic water (JJ101) on tissue repairing and anti-aging. In vitro, myogenic and antioxidant factors were determined in satellite cells cultured in JJ101 media. Satellite cells grown in JJ101 medium displayed increased expression of Notch1, Pax7, AMP-activating protein kinase-alpha (AMPK-alpha) and anti-oxidant enzymes and significant protection against hydrogen peroxide-induced cell death. In vivo, JJ101 enhanced muscle regeneration via increased number of regenerating muscle fibers and reduced muscle fibrosis. The lifespan in SMP30 KO mice fed with a VC-deficient diet was extended by drinking JJ101. Moreover, the hepatic expression of AMPK-α in JJ101-fed SMP30 KO mice was comparable to that of VC-fed SMP30 KO mice, whereas it was suppressed in tap water-fed SMP30 KO mice. These results suggest that bioactive ceramic water improves antioxidant status through up-regulating antioxidant enzymes and AMPK and thereby provides a favorable niche for satellite cells, leading to an increase in muscle regeneration and compensates oxidative stress.

ORGANISM(S): Mus musculus

PROVIDER: GSE80962 | GEO | 2020/12/01

REPOSITORIES: GEO

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