Dataset Information


Global expression data from HCT-8 human colorectal cancer (CRC) wild type cells (HCT8/WT) and its 5-FU-induced resistant cell line (HCT8/5-FU)

ABSTRACT: Complete growth medium RPMI-1640 which contains 10% FCS, 1% Penicillin-Streptomycin Solution was used for cell culture. Both cell lines were maintained at 37 °C in a humidified atmosphere containing 5% CO2. MTT assay was performed to determine the 5 ‑ FU resistance in both of HCT-8/WT and HCT-8/5-FU cell line. Overall design: CRC wild type cell (HCT8/WT) and its 5-FU-induced resistant cell line (HCT8/5-FU) , both of which were treated with 5-fluorouracil (5-FU) for 0, 24 and 48 hours, respectively. Three biological replicates of each condition were used for the microarray experiment.

INSTRUMENT(S): [PrimeView] Affymetrix Human Gene Expression Array

SUBMITTER: Guo Zheng  

PROVIDER: GSE81005 | GEO | 2017-03-15



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Epigenomics alternations and dynamic transcriptional changes in responses to 5-fluorouracil stimulation reveal mechanisms of acquired drug resistance of colorectal cancer cells.

Shen Y Y   Tong M M   Liang Q Q   Guo Y Y   Sun H Q HQ   Zheng W W   Ao L L   Guo Z Z   She F F  

The pharmacogenomics journal 20170103 1

A drug-induced resistant cancer cell is different from its parent cell in transcriptional response to drug treatment. The distinct transcriptional response pattern of a drug-induced resistant cancer cell to drug treatment might be introduced by acquired DNA methylation aberration in the cell exposing to sustained drug stimulation. In this study, we performed both transcriptional and DNA methylation profiles of the HCT-8 wild-type cells (HCT-8/WT) for human colorectal cancer (CRC) and the 5-fluor  ...[more]

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