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S. coelicolor YSK3225 R5- Medium Time-course Study (Culture #1)

ABSTRACT: Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarray methods to investigate the temporal transcriptome profiles of S. coelicolor A3(2) ∆absA1::apr mutant strain and compared with the M145 Wild-type strain (series submitted separately). Keywords: Time course

ORGANISM(S): Streptomyces coelicolor

PROVIDER: GSE8108 | GEO | 2007/06/14

SECONDARY ACCESSION(S): PRJNA100973

REPOSITORIES: GEO

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S. coelicolor YSK3225 R5- Medium Time-course Study (Culture #2)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarray methods to investigate the temporal transcriptome profiles of S. coelicolor A3(2) ∆absA1::apr mutant strain and compared with the M145 Wild-type strain (series submitted separately). Keywords: Time course

2007-06-14 | GSE8109 | GEO

S. coelicolor YSK4425 Time-course Study in R5- medium (Culture#2)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) afsS::apr mutant strain (YSK4425) and compare it to wild-type M145 strain. The regulatory protein encoded by afsS is known to affect antibiotic biosynthesis (Floriano, B., Bibb, M. 1996. afsR is a pleiotropic but conditionally required regulatory gene for antibiotic production in Streptomyces coelicolor A3(2). Mol Microbiol, 21, 385-96). Keywords: Time course

2007-06-23 | GSE8160 | GEO

S. coelicolor YSK3654 R5- medium time course study

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) deltaSCO3654::apr mutant strain (YSK3654) and compare it to the wild-type M145 strain. SCO3654 is a putative two-component system sensor kinase.

2010-10-25 | GSE21807 | GEO

S. coelicolor YSK3225 R5- Medium Time-course Study (Culture #1)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarray methods to investigate the temporal transcriptome profiles of S. coelicolor A3(2) âabsA1::apr mutant strain and compared with the M145 Wild-type strain (series submitted separately). Keywords: Time course Time-course study involved analysis of 12 samples (11h, 19h, 22h, 24h, 26h, 28h, 31h, 32h, 35h, 37h, 38h and 41h). All arrays used cDNA labeled with Alexa647 and gDNA labeled with Cy3.

2010-06-11 | E-GEOD-8108 | biostudies-arrayexpress

S. coelicolor YSK3225 R5- Medium Time-course Study (Culture #2)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarray methods to investigate the temporal transcriptome profiles of S. coelicolor A3(2) âabsA1::apr mutant strain and compared with the M145 Wild-type strain (series submitted separately). Keywords: Time course Time-course study involved analysis of 6 samples (18h, 24h, 26h, 28h, 32h and 42h). All arrays used cDNA labeled with Alexa647 and gDNA labeled with Cy3.

2010-06-10 | E-GEOD-8109 | biostudies-arrayexpress

S. coelicolor YSK4425 R5- Medium Time-course Study (Culture #1)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarray methods to investigate the temporal transcriptome profiles of S. coelicolor A3(2) ∆afsS::apr mutant strain and compared with the M145 Wild-type strain (series submitted separately). Keywords: Time course

2007-06-14 | GSE8110 | GEO

S. coelicolor M145 Time-course Study in R5- medium (Culture# 3)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) M145 wild type and disruption mutants of regulatory genes (afsS and absA1) known to affect antibiotic biosynthesis. Keywords: Time course

2007-06-19 | GSE8107 | GEO

S. coelicolor Wild Type Time-course Study in R5- medium (Culture#2)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) M145 wild type and disruption mutants of regulatory genes (afsS and absA1) known to affect antibiotic biosynthesis. Keywords: Time course

2007-06-19 | GSE8086 | GEO

S. coelicolor Wild Type Time-course Study in R5- medium (Culture#1)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) M145 wild type and disruption mutants of regulatory genes (afsS and absA1) known to affect antibiotic biosynthesis. Keywords: Time course

2007-06-19 | GSE8084 | GEO

S. coelicolor WL6268 R5- medium time course study

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) deltaSCO6268::kan mutant strain (WL6268) and compare it to the wild-type M145 strain. SCO6268 is a putative histidine kinase.

2010-10-25 | GSE21808 | GEO

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