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A transcript profile of Medicago truncatula 2HA and Jemmalong during somatic embryogenesis

ABSTRACT: The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared transcriptomes of tissue cultures from leaf explants of these two lines. We have used the Affymetrix Medicago Genome Array GeneChip to compare leaf explants of Medicago truncatula Jemalong and the superembryogenic line 2HA after two weeks of tissue culture on 10 µM 1-naphthaleneacetic acid (NAA) and 4 µM 6-benzylaminopurine (BAP) (P4 10:4) Keywords: Genotype comparison

ORGANISM(S): Medicago sativa Medicago truncatula Sinorhizobium meliloti

PROVIDER: GSE8131 | GEO | 2007/09/01

SECONDARY ACCESSION(S): PRJNA101015

REPOSITORIES: GEO

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Transcription profiling of Medicago truncatula 2HA and Jemmalong during somatic embryogenesis

Project description:The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared transcriptomes of tissue cultures from leaf explants of these two lines. We have used the Affymetrix Medicago Genome Array GeneChip to compare leaf explants of Medicago truncatula Jemalong and the superembryogenic line 2HA after two weeks of tissue culture on 10 ÂµM 1-naphthaleneacetic acid (NAA) and 4 ÂµM 6-benzylaminopurine (BAP) (P4 10:4) Experiment Overall Design: Leaf explants from the two genotypes were treated with 10:4 for two weeks; total RNA was extracted and used for hybridization to Affymetrix arrays

2008-06-16 | E-GEOD-8131 | biostudies-arrayexpress

A transcript profile of Medicago truncatula 2HA tissue culture with auxin treatment

Project description:Leaf explants of the superembryogenic Medicago truncatula line 2HA were treated with auxin (1-naphthaleneacetic acid) for one week to induce the formation of roots (Imin et al J Exp Bot 58:439-451). Gene expression in the leaves and the NAA treated tissue cultures was compared to identify transcripts expressed during the commitment to root formation in tissue culture. We have used the Affymetrix Medicago Genome Array GeneChip to compared gene expression in Medicago truncatula leaves and leaf explants that have been cultured for one week on NAA, to identify genes expressed during the commitment to root formation in tissue culture. Keywords: Cell type comparison

2007-10-01 | GSE8138 | GEO

Transcription profiling of Medicago truncatula 2HA tissue culture with auxin treatment

2008-06-17 | E-GEOD-8138 | biostudies-arrayexpress

Transcription profiling of Medicago truncatula with benzylaminopurine treatment to the root tips and untreated control plants

Project description:A BAP (benzylaminopurine, cytokinin) solution (10-7 M) was applied to root tips of Medicago truncatula (Jemalong A17 genotype) for 1h. Microarrays were used to compare the transcriptome of these plants (4 replicates) with the transcriptome of control plants, not exposed to BAP (4 replicates)

2010-01-01 | E-MEXP-1442 | biostudies-arrayexpress

Comparative proteomic analysis reveals differential root proteins in Medicago sativa and Medicago truncatula in response to salt stress

Project description:we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively.

2016-04-14 | PXD003761 | Pride

Expression data from Medicago truncatula roots treated with S. meliloti wild-type or exoA mutant bacteria, or auxin transport inhibitors

Project description:Publication title: Pseudonodule formation by wild type and symbiotic mutant Medicago truncatula in response to auxin transport inhibitors This SuperSeries is composed of the following subset Series: GSE27991: Expression data of Medicago truncatula Jemalong A17 roots treated with auxin transport inhibitors GSE28171: Expression data of Medicago truncatula Jemalong A17 roots treated with S. meliloti exoA mutant or auxin transport inhibitors GSE28172: Expression data of Medicago truncatula skl1-1 roots treated with S. meliloti wild-type or auxin transport inhibitors GSE28173: Genes differentially expressed in wild-type Medicago truncatula plants during nodulation Refer to individual Series

2011-03-25 | E-GEOD-28174 | biostudies-arrayexpress

Transcription profiling of root tips from Medicago truncatula Jemalong A17 and TN1.11 after salt stress

Project description:Effect of Salt Stress on root tip transcriptome of Medicago truncatula Jemalong A17 and Medicago truncatula TN1.11

2010-01-01 | E-MEXP-1255 | biostudies-arrayexpress

High-throughput sequencing of small RNAs in Medicago truncatula

2009-11-09 | GSE13761 | GEO

Transcription profiling of Medicago truncatula TTG1 over-expressing hairy root

Project description:Transcriptomics analysis of MtTTG1 over-expressing hairy root in the background of Medicago truncatula genotype Jemalong A17.

2009-08-14 | E-MEXP-1758 | biostudies-arrayexpress

High-throughput sequencing of small RNAs in Medicago truncatula

Project description:Medicago truncatula endogenous small RNAs The dataset contains Medicago truncatula Gaertn. cv. Jemalong endogenous small RNA sequences in the range 18-28 nucleotides. High-throughput Solexa/Illumina sequencing was carried out at the Sainsbury Laboratory, Norwich, UK. Please see www.illumina.com for details of the technology. Small RNA sequences were mapped to Medicago truncatula genome release 2.0 (http://www.medicago.org/genome/), the number of matches to the unfinished genome, if any, is recorded in the Series supplementary file GSE13761_sequence_annotations.txt.gz. Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

2009-11-09 | E-GEOD-13761 | biostudies-arrayexpress

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