Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Acetylation enhances TET2 function in protecting against abnormal DNA methylation during oxidative stress [A2780 damage time points - methylation]

ABSTRACT: Genome wide DNA methylation profiling of A2780 cells untreated (mock), treated with H2O2 for 30 min (H2O2 30 min), or treated with H2O2 for 30 min with additional 2.5 hour resting ( H2O2 3h) . The Illumina’s Infinium Human Methylation450 Beadchip Kit (WG-314-1001) was used to obtain DNA methylation profiles across approximately 450,000 CpGs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE81426 | GEO | 2016/12/31

SECONDARY ACCESSION(S): PRJNA321519

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Acetylation enhances TET2 function in protecting against abnormal DNA methylation during oxidative stress [A2780 TET2KD damage 3h - methylation]

Project description:Genome wide DNA methylation profiling of A2780 cells 1) infected with control virus, no H2O2 (Scr_mock), 2) infected with control virus with H2O2 treatment (30 min plus 2.5 h resting) (Scr_H2O2), 3) infected with shTET2 virus, no H2O2 (shTET2_mock), and 4) infected with shTET2 virus, with H2O2 treatment (30 min plus 2.5 h resting) (shTET2_H2O2, two biological replicates). The Illumina’s Infinium Human Methylation450 Beadchip Kit (WG-314-1001) was used to obtain DNA methylation profiles across approximately 450,000 CpGs.

2016-12-31 | GSE81427 | GEO

Acetylation enhances TET2 function in protecting against abnormal DNA methylation during oxidative stress [shTET2 - H2O2 - methylation]

Project description:Genome wide DNA methylation profilings (Bisulfite (BS), or oxidative bisulfite (oxBS)) were done for A2780 cells 1) infected with control virus, no H2O2 (Scr_mock) treatment, 2) infected with control virus with H2O2 treatment (30 min plus 2.5 h resting) (Scr_H2O2), and 3) infected with shTET2 virus, with H2O2 treatment (30 min plus 2.5 h resting) (shTET2_H2O2). Each genomic DNA was splitted equally to two aliquots. One aliquote was subjected to oxidation (oxBS) and one to mock oxidation (BS) prior to bisulfite treatment (CEGX protocol). The Illumina’s Infinium Human Methylation450 Beadchip Kit (WG-314-1001) was used to obtain DNA methylation profiles across approximately 450,000 CpGs. 5hmC levels were calculated by subtracting oxBS values from BS values.

2016-12-31 | GSE83750 | GEO

Acetylation enhances TET2 function in protecting against abnormal DNA methylation during oxidative stress [A2780 TET2 KO - methylation]

Project description:Genome wide DNA methylation profiling of A2780 control cells (Scr_1 and Scr_2), and A2780 TET2 KO clones (KO1.1, KO1.36, and KO2.32). The Illumina’s Infinium Human Methylation450 Beadchip Kit (WG-314-1001) was used to obtain DNA methylation profiles across approximately 450,000 CpGs.

2016-12-31 | GSE83751 | GEO

Evaluation of Inter-Batch Differences in Stem-Cell Derived Neurons

Project description:Genome wide DNA methylation profiling of Neurons derived from human induced pluripotent stem cells (iCell Neurons) at different time points were abtained by the Infinium HumanMethylation450 BeadChip (450K array) Kit (WG-314-1002), which interrogates >480,000 CpG sites in the human genome.In total, 36 samples were profiled using the 450K arrays. 1 ug purified DNA from each timepoint was submitted to the University of Chicago Genomics Core where it was converted with the EZ-96 DNA Methylationâ?¢ Kit (Deep Well Format), as per manufacturer's protocol, followed by array analysis using the Infinium HumanMethylation450 BeadChip (450K array) Kit (WG-314-1002), which interrogates >480,000 CpG sites in the human genome.

2015-12-15 | E-GEOD-66330 | biostudies-arrayexpress

Regulation of hydrogen peroxide-dependent gene expression in Rhodobacter sphaeroides: Regulatory functions of OxyR

Project description:Transcriptome profiling of Rhodobacter sphaeroides cells (WT and delta-oxyR mutant) grown in semiaerobic conditions, 7 and 30 min after addition of H2O2 up to 1 mM Keywords: time series

2005-06-22 | GSE2829 | GEO

Transcriptional profiling of the T. thermophilus HB8 response to hydrogen peroxide

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0, 5, 15 and 30 min after the addition of 10 mM H2O2.

2010-10-15 | GSE21430 | GEO

Evaluation of Inter-Batch Differences in Stem-Cell Derived Neurons

2015-12-15 | GSE66330 | GEO

DNAmethylation_array_data_of_PSC

Project description:DNA methylation data for Pulmonary Sarcomatoid Carcinoma (PSC) patients were generated using the Infinium MethylationEPIC Kit (Illumina, cat. no. WG-317-1001) or HumanMethylation450 BeadChip Kit (Illumina, cat. no. WG-314-1003).

| OEX003903 | NODE

DNAmethylation_array_data_of_PSC

| OEX003904 | NODE

Saccharomyces cerevisiae response to various stressors

Project description:Samples GSM206658-GSM206693: Acquired Stress resistance in S. cerevisiae: NaCl primary and H2O2 secondary Transcriptional timecourses of yeast cells exposed to 0.7M NaCl alone, 0.5mM H2O2 alone, or 0.5mM H2O2 following 0.7M NaCl, all compared to an unstressed sample. Repeated using msn2∆ strain. Samples GSM291156-GSM291196: Transcriptional response to stress in strains lacking MSN2 and/or MSN4 Transcriptional timecourses of yeast cells (WT, msn2∆, msn4∆, or msn2∆msn4∆) exposed to 0.7M NaCl for 45 minutes or 30-37˚C Heat Shift for 15 min compared to an unstressed sample of the same strain. Keywords: Stress Response

2008-09-05 | GSE8335 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data