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Transcriptional profiling of the maturation of human in vivo-generated plasma cells

ABSTRACT: In vivo antigen (Ag)-induced differentiation of B lymphocytes into plasma cells (PCs) takes place in extra-follicular foci and germinal centers of the secondary lymphoid organs (SLOs). Most of these SLO PCs are short-living and only relatively few PCs, characterized by secreting high-affinity antibodies (Ab), travel through the circulation and finally home in specialized survival niches of the bone marrow (BM) and, at a lesser extent, in the SLOs, where they become long-living Ab-secreting PCs. Initially, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish between human circulating Ag-induced PCs in comparison with tonsil and BM PCs distinctively regulate genes involved in cell proliferation. Now, moreover, to characterize further the B-cell transition into PC, transcriptomes from human naïve B lymphocytes were also included in the analysis, and genes showing significant changes in the comparison between B lymphocytes and every of the three PC subsets were selected as the PC signature.

ORGANISM(S): Homo sapiens

PROVIDER: GSE81589 | GEO | 2017/09/12

SECONDARY ACCESSION(S): PRJNA322053

REPOSITORIES: GEO

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Gene expression signatures of human plasma cells isolated from different territories

Project description:In vivo antigen (Ag)-induced differentiation of B lymphocytes into plasma cells (PCs) takes place in extra-follicular foci and germinal centers of the secondary lymphoid organs (SLOs). Most of these SLO PCs are short-living and only relatively few PCs, characterized by secreting high-affinity antibodies (Ab), travel through the circulation and finally home in specialized survival niches of the bone marrow (BM) and, at a lesser extent, in the SLOs, where they become long-living Ab-secreting PCs. We have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish between human circulating Ag-induced PCs in comparison with tonsil and BM PCs distinctively regulate genes involved in cell proliferation.

2015-01-05 | GSE59697 | GEO

Gene expression signatures of human plasma cells isolated from different territories

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Tumor cells in light-chain amyloidosis and multiple myeloma show different transcriptional rewiring of the normal plasma cell development [single-cell RNA-seq]

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Network analysis identifies proinflammatory plasma cell polarization for secretion of ISG15 in human autoimmunity

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2016-07-22 | E-GEOD-75007 | biostudies-arrayexpress

Network analysis identifies proinflammatory plasma cell polarization for secretion of ISG15 in human autoimmunity

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Single-cell RNA sequencing reveals tissue-associated fibroblastic reticular cell heterogeneity in human secondary lymphoid organs

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Mature IgM+ plasma cells sense antigen and develop competence for cytokine production upon antigenic challenge

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2016-11-01 | GSE80216 | GEO

Gene expression profile at single cell level of plasma cells (PCs) from human bone marrow

Project description:Antibody-secreting plasma cells (PCs) are generated in secondary lymphoid organs, but are reported to reside in an emerging range of anatomical sites. Analysis of the transcriptome of different tissue-resident (Tr)PC populations revealed that they each have their own transcriptional signature indicative of functional adaptation to the host tissue environment. In contrast to expectation, all TrPCs were extremely long-lived, regardless of their organ of residence, with the longevity influenced by intrinsic factors like the immunoglobulin isotype. Analysis at single-cell resolution revealed that the bone marrow is unique in housing a compendium of PCs generated all over the body that retain aspects of the transcriptional program indicative of their tissue of origin.

2023-11-12 | GSE229957 | GEO

Chemically Induced Paneth Cells Provide Superior In Vivo Mimic to Organoid-derived Analog

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2018-04-02 | GSE100274 | GEO

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