Genomics

Dataset Information

32

Distinct Functions of Argonaute Slicer in siRNA Maturation and Heterochromatin Formation


ABSTRACT: Small-RNA (sRNA)-guided transcriptional gene silencing by Argonaute (Ago)-containing complexes is fundamental to genome integrity and epigenetic inheritance. The RNA cleavage (“Slicer”) activity of Argonaute has been implicated in both sRNA maturation and target RNA cleavage. Typically, Argonaute slices and releases the passenger strand of duplex sRNA to generate active silencing complexes, but it remains unclear whether slicing of target nascent RNAs, or other RNAi components, also contributes to downstream transcriptional silencing. Here, we develop a strategy for loading the fission yeast Ago1 with a single-stranded sRNA guide, which bypasses the requirement for slicer activity in generation of active silencing complexes. We show that slicer-defective Ago1 can mediate secondary sRNA generation, H3K9 methylation, and silencing similar to or better than wild-type and associates with chromatin more efficiently. The results define an ancient and minimal sRNA-mediated chromatin silencing mechanism, which resembles the germline-specific sRNA-dependent transcriptional silencing pathways in Drosophila and mammals. This SuperSeries is composed of the SubSeries listed below. Overall design: Refer to individual Series

INSTRUMENT(S): Illumina HiSeq 2000 (Schizosaccharomyces pombe)

SUBMITTER: Nahid Iglesias  

PROVIDER: GSE81734 | GEO | 2016-12-19

SECONDARY ACCESSION(S): GSE81732 GSE81731 GSE81733PRJNA322453

REPOSITORIES: GEO

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Publications

Distinct Functions of Argonaute Slicer in siRNA Maturation and Heterochromatin Formation.

Jain Ruchi R   Iglesias Nahid N   Moazed Danesh D  

Molecular cell 20160707 2


Small-RNA (sRNA)-guided transcriptional gene silencing by Argonaute (Ago)-containing complexes is fundamental to genome integrity and epigenetic inheritance. The RNA cleavage ("Slicer") activity of Argonaute has been implicated in both sRNA maturation and target RNA cleavage. Typically, Argonaute slices and releases the passenger strand of duplex sRNA to generate active silencing complexes, but it remains unclear whether slicing of target nascent RNAs, or other RNAi components, also contributes  ...[more]