Project description:We report gene expression profiling in the fission yeast Schizosaccharomyces pombe. We performed high-throughput sequencing of RNA isolated from wild-type, mmi1(W112A) mutant, erh1∆, and mmi1∆ strains. We find that mmi1(W112A) and erh1∆ derepress a shared subset of mmi1-repressed genes during vegetative growth.
Project description:We explored transcriptional profiles of the fission yeast Schizosaccharomyces pombe. RNA-seq was used to characterize changes in expression profiles of all known lncRNAs and mRNAs in wild type cells.
Project description:This RNA-Seq analysis compares gene expression of the vtc4∆ mutant contrasting to the wild-type fission yeast (Schizosaccharomyces pombe).
Project description:RNA-seq analysis compares gene expression of the ecl3∆ and ecl123∆ mutants to the wild-type fission yeast (Schizosaccharomyces pombe).
Project description:This RNA-Seq analysis compares gene expression of the wild-type fission yeast (Schizosaccharomyces pombe) strain at various times after transfer to defined phosphate-replete ePMGT(+PO4) medium (2, 4, 8 HR) contrasting to the WT fission yeast S. pombe cells grown in phosphate-replete YES medium (0HR).
Project description:The aim was to compare the levels of histone H3 dimethylation at lysine 9 (H3K9me2) in wild-type and dbl2 knock-out Schizosaccharomyces pombe cells. Fission yeast cultures were grown in the complex YES medium to exponential phase and chromatin immunoprecipitation was carried out using anti-H3 and anti-H3K9me2 antibodies. Two independent biological replicates were performed. The resulting IP and input DNA samples were sequenced using Illumina sequencing (35 nt PE). H3K9me2 occupancy in each sample was normalized to the corresponding total H3 occupancy, and normalized H3K9me2 levels were compared between WT and dbl2 knock-out.
Project description:We report gene expression profiling in the fission yeast Schizosaccharomyces pombe. We performed high-throughput sequencing of RNA isolated from wild-type, erh1∆, and ccr4∆ strains. We find that many meiotic gene containing degradation sequence DSR are expressed in vegetative erh1∆, while these meiotic mRNAs do not increase in ccr4∆, indicating that Erh1 and Ccr4 target different set of genes during vegetative growth.