Comparative Total RNA-seq between WT and alba4-null Plasmodium yoelii parasites

ABSTRACT: To determine the role of ALBA4 in mRNA homeostasis in Plasmodium parasites, we performed comparative total RNA-seq between a WT-GFP line and an alba4-null line. We performed these experiments at three points in the life cycle - asexual (schizont), sexual (gametocyte), and oocyst sporozoite stages. We found that ALBA4 has a multi-faceted role in mRNA homeostasis, and is involved in mRNA fate determination. ALBA4 appears to act in a stage-specific manner, affecting different sets of transcripts in each stage. Our data suggests that ALBA4 plays opposing roles - possibly promoting degradation in schizonts, while promoting mRNA protection in gametocytes. In oocyst sporozoites, it appears that ALBA4 has a mixed role, and may be involved in both degradation and protection of mRNAs.

ORGANISM(S): Plasmodium yoelii

PROVIDER: GSE81834 | GEO | 2017/08/25

SECONDARY ACCESSION(S): PRJNA322665

REPOSITORIES: GEO