Transcriptomics,Genomics

Dataset Information

22

Tet-Gabpα cKO ES cells: -Tet vs. +Tet


ABSTRACT: We generated tetracycline-inducible myc-GABPα-expressing Gabpα conditional knockout embryonic stem cells (Tet-Gabbpα cKO ES cells), and then compared the transcriptional profiles between control (-Tet) and Gabpα-null (+Tet) cells by an oligo DNA microarray analysis. Overall design: Tet-Gabpα cKO ES cells were cultured with or without Tet for 1 day. Total RNAs were isolated and subjected to microarray analysis.

INSTRUMENT(S): 3D-Gene Mouse Oligo chip 24k

SUBMITTER: Satoshi Kondo  

PROVIDER: GSE82231 | GEO | 2017-07-01

SECONDARY ACCESSION(S): PRJNA324401

REPOSITORIES: GEO

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Publications

GA-Binding Protein Alpha Is Involved in the Survival of Mouse Embryonic Stem Cells.

Ueda Atsushi A   Akagi Tadayuki T   Yokota Takashi T  

Stem cells (Dayton, Ohio) 20170808 11


Ets-related transcription factor GA-binding protein alpha (GABPα), which is encoded by Gabpa, is expressed in a variety of cell types and is involved in cellular functions such as cell cycle regulation, apoptosis, and differentiation. Here, we generated Gabpa conditional knockout embryonic stem cells (ESCs) and characterized its cellular phenotypes. Disruption of Gabpa revealed that the proliferation of Gabpa-null ESCs was drastically repressed and cells started to die within 2 days. The repress  ...[more]

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